• Introduction and General Properties of Plasmids
• 31 min
  1. Introduction to plasmid biology

  • Prof. Emeritus Don Clewell
• 39 min
  2. General properties and applications of plasmids

  • Prof. Emeritus Don Clewell
• Replication
• 47 min
  3. Plasmids: copy number control by antisense RNAs

  • Prof. Gerhart Wagner
• 30 min
  4. Plasmid rolling-circle (RC) replication

  • Prof. Saleem Khan
• 45 min
  5. Replication of linear plasmids in bacteria

  • Prof. George Chaconas
• Maintenance and Partitioning
• 32 min
  6. Plasmid segregation and stability in bacteria

  • Prof. Barbara Funnell
• 33 min
  7. Plasmid stabilization by cell killing

  • Prof. Keith Weaver
• 30 min
  8. Dimer catastrophes and plasmid resolution

  • Dr. David Summers
• Conjugation and Mobilization
• 41 min
  9. The diversity of bacterial conjugation and mobilization systems

  • Prof. Fernando de la Cruz
• 36 min
  10. The pCF10-encoded pheromone response: cell-cell signaling, complexity and evolution

  • Prof. Gary Dunny
• 27 min
  11. DNA processing in conjugative transfer

  • Dr. Joel Schildbach
• 43 min
  12. Agrobacterium tumefaciens

  • Dr. Stephen Winans
• Genetic Traits Frequently Associated with Plasmids
• 37 min
  13. Insertion sequences and DNA transposition

  • Prof. William Reznikoff
• 48 min
  14. Plasmids, integrons and the spread of antibiotic resistance

  • Prof. Hatch Stokes
• 30 min
  15. Virulence plasmids

  • Prof. Julian Rood
• Eukaryotic Plasmids
• 30 min
  16. The yeast plasmid: a highly persistent selfish DNA element

  • Prof. Makkuni Jayaram
• Population Biology and Evolution
• 35 min
  17. Evolution of plasmids and their role in bacterial diversity and adaptability

  • Prof. Christopher Thomas
• Archived Lectures *These may not cover the latest advances in the field
• 85 min
  18. Bacterial extrachromosomal elements: role in chemical detoxification and potential cancer therapy

  • Prof. Ananda Chakrabarty
• 34 min
  19. Plasmid segregation and stability in bacteria

  • Prof. Barbara Funnell
• 39 min
  20. Replication of linear plasmids in bacteria

  • Prof. George Chaconas

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Plasmids
3. Conjugative plasmids
4. Lederberg and Tatum experiments
5. Bacterial conjugation
6. Conjugative transfer requirements
7. Processes required for transfer
8. Example: F factor
9. DNA processing in F conjugative transfer
10. Transfer genes on F factor
11. F plasmid oriT
12. F plasmid tra operon
13. TraY and IHF
14. F plasmid oriT and TraI
15. Model for F factor transfer initiation
16. Molecular mechanisms and regulation
17. F oriT near nic
18. DNA form pre- and post-cleavage
19. Evidence for context-dependent TraI recognition
20. Other questions
21. References

Topics Covered

• Conjugative plasmids
• Original observation of conjugative transfer
• Description of transfer
• DNA specificity in conjugative transfer
• F transfer as an example
• Roles of F proteins in transfer
• Context dependence of F TraI recognition as regulation

Links

Series:

• Biology and Significance of Plasmids

Categories:

• Genetics & Epigenetics
• Methods
• Microbiology

Talk Citation

Publication History

• Published on January 28, 2008

Financial Disclosures

• Dr. Joel Schildbach has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.