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Welcome everyone to Chapter 4 of this short talk series on nuclear receptors as therapeutic targets. In Chapter 4 and the consecutive Chapter 5, we will have a look at the structural and the molecular mechanisms of nuclear receptor activity, and we will start in Chapter 4 with the mechanisms of DNA interaction and the role of dimerization.
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In Chapter 2, we have seen the classification of nuclear receptors based on their cellular localization and their dimerization. Remember that the steroid hormone receptors of the NR3 family here on the left are Type I nuclear receptors and are localized in the cytosol, bound to heat shock proteins in the absence of a ligand. Upon agonist binding, the receptor dissociates from the heat shock protein, forms a homodimer, and translocates to the nucleus to bind to the DNA. The other nuclear receptors belonging to the Type II, III, and IV, in contrast, are typically localized in the nucleus and bound to DNA also in the absence of ligands. They act in different mono or oligomeric forms, depending on what type they are, and they often form heterodimers with RXR or homodimers or monomers. It should be noted that several nuclear receptors of this Type II, III, and IV can act in more than one of these forms, so the association to Type II, III, and IV is not always exact.
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Here you see an overview of the nuclear receptor family with the receptors labeled according to their DNA interaction classification as Type I, II, III, or IV. Only steroid hormone receptors in the middle and labeled in violet belong to Class I, which translocate to the nucleus after ligand binding, and then act as homodimers. The majority of nuclear receptors in the NR1 family on the left, mainly act as heterodimers with a retinoid X receptor, abbreviated as RXR, and they are labeled in blue, and some of them can additionally act as a monomer. revERB receptors and retinoic acid receptor-related orphan receptors also belonging to the NR1 family on the left and labeled in red, typically act as monomers or homodimers. The NR2 and NR4 families, labeled orange, can be active in all three forms, whereas, NR5 receptors, labeled in green, act only as monomers, and NR6, in brown, only form homodimers, according to current understanding. The three RXRs, labeled in light blue in the middle, are the universal heterodimer partner for other nuclear receptors, and the NR0 receptors, DAX1 and SHP, on the upper right lack a DNA binding domain as we have seen, so they are not included in the classification according to DNA interaction type.

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Mechanisms and molecular function of nuclear receptors 1

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