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2. Toll-like receptor signalling during infection and inflammation
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3. Cytokines and CD4 T Cells: dance partners at the immunology ball
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4. The gamma c family of cytokines
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5. Interleukin-6: back to the future
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6. Regulation of the immune response to pathogens 1
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7. Regulation of the immune response to pathogens 2
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8. IL-17 cytokine family and receptors
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15. Chemokines and their receptors: their biology and therapeutic relevance
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17. Humoral innate immunity and the acute phase response 2
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18. Receptor identification for alarmins and DAMPs
- Dr. Joost J. Oppenheim
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19. Assays for cytokines and cytokine assay performance 1
- Prof. Theresa Whiteside
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20. Assays for cytokines and cytokine assay performance 2
- Prof. Theresa Whiteside
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21. Interleukin-7 and T cells
- Dr. Scott Durum
- Archived Lectures *These may not cover the latest advances in the field
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22. Assays for cytokines and cytokine assay standardization
- Prof. Theresa Whiteside
Printable Handouts
Navigable Slide Index
- Introduction
- Measurements of cytokines
- Features relevant to assay development
- Types of assays for cytokines
- Ex vivo assays for cytokines
- The principle of ELISA
- Assay selection is a critical step
- Single-cell assays for intracellular expression
- Intracytoplasmic cytokine staining (ICS)
- "Cytokine screen" with PBMC
- Enumeration of cytokine-secreting single T cells
- Immunohistochemistry for TGF-beta1
- Multiplex analyses
- Multiplex bead array technology
- How the Bio-Plex protein array system works
- Cytokine profiling by xMAP
- ELC platform
- Protein expression or cytokine transcripts?
- QC/QA issues: general principles
- Good laboratory practice: QC
- IL-12p70 production by human dendritic cells
- Comparing commercial/in-house IL-12 standards
- Data analysis in cytokine assays
- The SPICE program
- IL-12p70 levels established for DC products
- Pitfalls of cytokine assays
- Cytokines in biotherapy trials
- Acknowledgement
Topics Covered
- The importance of measuring cytokines
- The types of cytokine assays available
- Examples of most widely used cytokine assays
- Principles of ELISA and ELISPOT
- How to select the right cytokine assay
- Multiplex analyses and cytokine profiling
- Principles of QA/QC for cytokine assays
- Data analysis and pitfalls
Talk Citation
Whiteside, T. (2011, December 5). Assays for cytokines and cytokine assay standardization [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 3, 2025, from https://doi.org/10.69645/KSZI7822.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Theresa Whiteside has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.