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• 52 min
  1. Principles of cytokine-receptor interactions and signal transduction

  • Dr. Xiaoxia Li
• 47 min
  2. Toll-like receptor signalling during infection and inflammation

  • Prof. Luke O'Neill
• 74 min
  3. Cytokines and CD4 T Cells: dance partners at the immunology ball

  • Dr. William E. Paul
• 38 min
  4. The gamma c family of cytokines

  • Dr. Warren J. Leonard
• 29 min
  5. Interleukin-6: back to the future

  • Prof. Tadamitsu Kishimoto
• 59 min
  6. Regulation of the immune response to pathogens 1

  • Prof. Anne O’Garra
• 33 min
  7. Regulation of the immune response to pathogens 2

  • Prof. Anne O’Garra
• 23 min
  8. IL-17 cytokine family and receptors

  • Prof. Chen Dong
  • Dr. Seon Hee Chang
• 40 min
  9. The b common (bc) family of cytokines, receptors and ligands

  • Prof. Angel Lopez
  • Dr. Timothy Hercus
• 29 min
  10. TNF superfamily cytokines and receptors in the healthy and diseased immune system

  • Dr. Richard Siegel
• 76 min
  11. An introduction to the interleukin-1 family of cytokines

  • Dr. John Sims
• 49 min
  12. IL-1 as a key inflammatory regulator in autoinflammatory diseases

  • Dr. Raphaela Goldbach-Mansky
• 58 min
  13. Signaling for the interferon family of receptors and ligands and clinical implications

  • Prof. Leonidas Platanias
• 45 min
  14. Interferons as antivirals: translating basic research into clinical application

  • Prof. Eleanor N. Fish
• 49 min
  15. Chemokines and their receptors: their biology and therapeutic relevance

  • Dr. Amanda Proudfoot
• 22 min
  16. Humoral innate immunity and the acute phase response 1

  • Prof. Alberto Mantovani
• 42 min
  17. Humoral innate immunity and the acute phase response 2

  • Prof. Alberto Mantovani
• 49 min
  18. Receptor identification for alarmins and DAMPs

  • Dr. Joost J. Oppenheim
• 41 min
  19. Assays for cytokines and cytokine assay performance 1

  • Prof. Theresa Whiteside
• 39 min
  20. Assays for cytokines and cytokine assay performance 2

  • Prof. Theresa Whiteside
• 23 min
  21. Interleukin-7 and T cells

  • Dr. Scott Durum
• Archived Lectures *These may not cover the latest advances in the field
• 32 min
  22. Assays for cytokines and cytokine assay standardization

  • Prof. Theresa Whiteside

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Measurements of cytokines
3. Features relevant to assay development
4. Types of assays for cytokines
5. Ex vivo assays for cytokines
6. The principle of ELISA
7. Assay selection is a critical step
8. Single-cell assays for intracellular expression
9. Intracytoplasmic cytokine staining (ICS)
10. "Cytokine screen" with PBMC
11. Enumeration of cytokine-secreting single T cells
12. Immunohistochemistry for TGF-beta1
13. Multiplex analyses
14. Multiplex bead array technology
15. How the Bio-Plex protein array system works
16. Cytokine profiling by xMAP
17. ELC platform
18. Protein expression or cytokine transcripts?
19. QC/QA issues: general principles
20. Good laboratory practice: QC
21. IL-12p70 production by human dendritic cells
22. Comparing commercial/in-house IL-12 standards
23. Data analysis in cytokine assays
24. The SPICE program
25. IL-12p70 levels established for DC products
26. Pitfalls of cytokine assays
27. Cytokines in biotherapy trials
28. Acknowledgement

Topics Covered

• The importance of measuring cytokines
• The types of cytokine assays available
• Examples of most widely used cytokine assays
• Principles of ELISA and ELISPOT
• How to select the right cytokine assay
• Multiplex analyses and cytokine profiling
• Principles of QA/QC for cytokine assays
• Data analysis and pitfalls

Links

Series:

• Cytokines

Categories:

• Immunology
• Methods

Therapeutic Areas:

• Immunology & Inflammation

Talk Citation

Publication History

• Published on December 5, 2011
• Archived on January 31, 2022

Financial Disclosures

• Prof. Theresa Whiteside has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.