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1. Introduction
2. Proteomics
3. Rationale for accurate proteome quantification
4. Quantitative proteomics - stable isotope labeling
5. Gel free proteome analytical platform
6. Protein identification strategy
7. Accurate quantitation using isotope dilution
8. Isotope coded affinity tags (ICAT)
9. Quantitative proteomics by stable isotope dilution
10. Stable isotope labeling strategies
11. Quantitative proteomics technology
12. Selective isolation from complex mixtures
13. Quantitative proteomics via stable isotope tagging
14. Label-free quantitative proteomics
15. LC-MS label free proteomics method
16. LC-MS data
17. Reproducibility of LC-MS data
18. SuperHirn and MasterMaps
19. Feature map of four protein mixture
20. Feature map of five protein mixture
21. Feature map of protein no. 5
22. LC-MS results example 1
23. LC-MS results example 2
24. LC-MS results example 3
25. LC-MS approach to sample profiling
26. Calibrated reference peptide probes
27. Sequential proteomic experiments
28. PeptideAtlas: different builds
29. Targeted proteomics paradigm
30. Multiple reaction monitoring (MRM)
31. MRM quantification (internal standards)
32. Duty cycle
33. Scheduled MRMs
34. Summary
35. Conclusions

Topics Covered

• Proteomics
• Quantitative mass spectrometry
• Stable isotope labelling
• Label free protein quantification
• PeptideAtlas project
• Targeted mass spectrometry via multiple reaction monitoring

Links

Series:

• Chemical Biology

Categories:

• Biochemistry
• Methods

Talk Citation

Publication History

• Published on August 14, 2008

Financial Disclosures

• Prof. Ruedi Aebersold has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.