• Architecture of the ribosome RNA-protein machine assembly
• 49 min
  1. High resolution structure of ribosomal subunits RNA/RNA and protein/RNA interactions

  • Prof. Nenad Ban
• 38 min
  2. Assembly of the 30S ribosomal subunit in vitro and in cells

  • Prof. James Williamson
• Decoding and peptide bond formation
• 46 min
  3. How aminoacyl-tRNA synthetases translate the genetic code

  • Dr. Stephen Cusack
• 42 min
  4. The structural basis for how the large ribosomal subunit catalyses peptide bond formation

  • Prof. Thomas Steitz
• 47 min
  5. Elongation of protein synthesis: structural basis of the process of decoding

  • Prof. Marina Rodnina
• Initiation of protein synthesis
• 49 min
  6. The structure-based mechanism of translation initiation in bacteria and eukaryotes

  • Prof. Jeffrey Kieft
• 48 min
  7. Factor independent initiation of protein synthesis by IRES RNAs

  • Prof. Jeffrey Kieft
• Elongation and termination of protein synthesis
• 41 min
  8. Translocation: movement of tRNA and mRNA through the ribosome

  • Prof. Harry Noller
• 31 min
  9. Recoding: getting more out of the message by shifting reading frame and redefining codon meaning

  • Prof. John Atkins
  • Prof. Raymond Gesteland
• Co-translational protein secretion
• 75 min
  10. Mechanism of translocon function: current insights and models

  • Prof. Arnold Driessen
• How antibiotics target the ribosome
• 29 min
  11. Antibiotics that target the 50S ribosome subunit: resistance and other considerations

  • Dr. Joyce Sutcliffe
• 35 min
  12. Antibiotic inhibition of ribosome function

  • Dr. Daniel Wilson
• 45 min
  13. Structure-based drug design targeting infectious disease

  • Dr. Erin Duffy
• Archived Lectures *These may not cover the latest advances in the field
• 26 min
  14. Structural insights into aminoacyl-tRNA delivery by EF-Tu and translocation by EF-G

  • Prof. Joachim Frank
• 21 min
  15. Introduction to the ribosome

  • Prof. Anders Liljas
• 20 min
  16. The structure of the intact ribosome and ribosomal subunit interactions

  • Dr. Jamie H. Doudna Cate
• 42 min
  17. Structural insights into decoding of mRNA by the ribosome

  • Prof. Venki Ramakrishnan
• 63 min
  18. Chemistry of peptide bond formation

  • Prof. Rachel Green
• 49 min
  19. The interaction of antibiotics with the large ribosomal subunit from Haloarcula marismortui

  • Prof. Peter Moore

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. The 50S ribosomal subunit: a unique drug target
3. Why target the 50S ribosomal subunit?
4. Analysis of antibacterial market
5. Target markets
6. Drug resistance mechanisms (1)
7. Drug resistance mechanisms (2)
8. Intracellular vs. extracellular pathogens
9. Sequestration: MRSA abscess
10. Sequestration: biofilm development
11. Cell surfaces of Gram-positive/negative bacteria
12. How do antibiotics gain entry into cells?
13. Porins
14. Lack of uptake: streptogramin B
15. Self-promoted uptake: azithromycin
16. Drug modification: inactivation
17. Target modifications
18. Erm genes of bacteria and producer strains
19. 23S mutations conferring linezolid resistance
20. 23S mutations conferring macrolide resistance
21. Target modifications: rRNA operons
22. Target mutations: ribosomal proteins
23. Drug efflux
24. Major superfamilies of transport proteins
25. Illustration of drug efflux through transporters
26. General considerations

Topics Covered

• Why target the 50S ribosomal subunit?
• Analysis of antibacterial market
• Mechanisms of drug resistance
• Sequestration
• How do antibiotics gain entry into cells
• Porins
• Lack of uptake
• Self-promoted uptake drug modification
• Target modifications
• Drug efflux
• Major superfamilies of transport proteins

Links

Series:

• The Mechanisms of Ribosome Function

Categories:

• Biochemistry
• Microbiology
• Pharmaceutical Sciences

Therapeutic Areas:

• Infectious Diseases

Talk Citation

Publication History

• Published on May 15, 2008

Financial Disclosures

• Dr. Joyce Sutcliffe has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.