• Architecture of the ribosome RNA-protein machine assembly
• 49 min
  1. High resolution structure of ribosomal subunits RNA/RNA and protein/RNA interactions

  • Prof. Nenad Ban
• 38 min
  2. Assembly of the 30S ribosomal subunit in vitro and in cells

  • Prof. James Williamson
• Decoding and peptide bond formation
• 46 min
  3. How aminoacyl-tRNA synthetases translate the genetic code

  • Dr. Stephen Cusack
• 42 min
  4. The structural basis for how the large ribosomal subunit catalyses peptide bond formation

  • Prof. Thomas Steitz
• 47 min
  5. Elongation of protein synthesis: structural basis of the process of decoding

  • Prof. Marina Rodnina
• Initiation of protein synthesis
• 49 min
  6. The structure-based mechanism of translation initiation in bacteria and eukaryotes

  • Prof. Jeffrey Kieft
• 48 min
  7. Factor independent initiation of protein synthesis by IRES RNAs

  • Prof. Jeffrey Kieft
• Elongation and termination of protein synthesis
• 41 min
  8. Translocation: movement of tRNA and mRNA through the ribosome

  • Prof. Harry Noller
• 31 min
  9. Recoding: getting more out of the message by shifting reading frame and redefining codon meaning

  • Prof. John Atkins
  • Prof. Raymond Gesteland
• Co-translational protein secretion
• 75 min
  10. Mechanism of translocon function: current insights and models

  • Prof. Arnold Driessen
• How antibiotics target the ribosome
• 29 min
  11. Antibiotics that target the 50S ribosome subunit: resistance and other considerations

  • Dr. Joyce Sutcliffe
• 35 min
  12. Antibiotic inhibition of ribosome function

  • Dr. Daniel Wilson
• 45 min
  13. Structure-based drug design targeting infectious disease

  • Dr. Erin Duffy
• Archived Lectures *These may not cover the latest advances in the field
• 26 min
  14. Structural insights into aminoacyl-tRNA delivery by EF-Tu and translocation by EF-G

  • Prof. Joachim Frank
• 21 min
  15. Introduction to the ribosome

  • Prof. Anders Liljas
• 20 min
  16. The structure of the intact ribosome and ribosomal subunit interactions

  • Dr. Jamie H. Doudna Cate
• 42 min
  17. Structural insights into decoding of mRNA by the ribosome

  • Prof. Venki Ramakrishnan
• 63 min
  18. Chemistry of peptide bond formation

  • Prof. Rachel Green
• 49 min
  19. The interaction of antibiotics with the large ribosomal subunit from Haloarcula marismortui

  • Prof. Peter Moore

Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Foundmental processes during protein synthesis
3. Peptidyl transfer catalysis by RNA-rich active site
4. Ribosome structure solution provided new insights
5. Peptide bond formation and peptide release
6. Active site of the ribosome
7. Universally conserved nucleotides of the active site
8. Affinity tag purification of mutant ribosomes
9. Pre-steady state assay for peptide bond formation
10. Active site mutations and rates of peptidyl transfer
11. A-site substrates: tRNA vs. puromycin
12. Active site variants recovery by C75 addition to Pm
13. "Uninduced" state of ribosome and P-site substrate
14. A-site substrates-induced conformational changes
15. Peptide release activity of variant ribosomes
16. How do release factors function?
17. RF catalysis in the large subunit
18. Other RF conserved amino acids in bacteria
19. Consequences of RF1 site-directed mutations
20. How can we make substitution in water?
21. Nucleophile partitioning provides a handle
22. Mutants that appear to have lost water specificity
23. Permissivity and size of side chain at position 235
24. RF components functioning in activating hydrolysis
25. Summary
26. Regulation of translation post-initiation

Topics Covered

• Fundamental processes during protein synthesis
• Peptidyl transfer catalysis by RNA-rich active site
• Ribosome structure solution provided new insights
• Peptide bond formation and peptide release
• Active site of the ribosome
• Universally conserved nucleotides of the active site
• Affinity tag purification of mutant ribosomes
• Active site mutations and rates of peptidyl transfer
• A-site substrates: tRNA vs. puromycin
• "Uninduced" state of ribosome and P-site substrate
• A-site substrates-induced conformational changes
• Peptide release activity of variant ribosomes
• RF catalysis in the large subunit
• RF conserved amino acids in bacteria
• Consequences of RF1 site-directed mutations
• Nucleophile partitioning
• Mutants that lost water specificity
• RF components functioning in activating hydrolysis
• Regulation of translation post-initiation

Links

Series:

• The Mechanisms of Ribosome Function

Categories:

• Biochemistry

Talk Citation

Publication History

• Published on May 15, 2008
• Archived on August 13, 2020

Financial Disclosures

• Prof. Rachel Green has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.