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- Introduction
- Mechanisms to produce biological responses
- Optimal ligand shapes
- Modes of action of 1alpha,25(OH)2D3
- What are the different ligand shapes?
- Shape of ligand for VDRnuc
- Three dimensional shape of the VDR
- 6-s-trans shape of the ligand for the VDR
- Structural basis for binding to human DBP
- DBP structure: domains A, B, C
- Shape of the ligand for DBP
- Shape of the ligand for rapid responses (1)
- Shape of the ligand for rapid responses (2)
- Summary of optimal ligand shapes
- Genomic responses
- Steroid receptor superfamily
- Gene family of nuclear receptors for small ligands
- Steroid receptor domains
- Organization of VDR gene, mRNA and protein
- Model of steroid hormone mode of action
- Nuclear receptor zinc fingers
- 1alpha,25(OH)2D3 transcriptional activation
- Determination of VDR-mediated genomic response
- Summary of genomic response
- Rapid responses
- Reminder: modes of action of 1alpha,25(OH)2D3
- Examples of rapid responses
- Molecular tools
- Ion current potentiation by 1,25D3 in osteoblasts
- Effect of 1beta,25(OH)2D3 on Cl-channels opening
- Structure-function studies on 1alpha,25(OH)2D3
- Structure-function analysis of rapid responses
- Caveolae
- Isolation of caveolae membrane fractions
- [3H]-1alpha,25(OH)2D3 binding to CMF
- Additional caveolae analyses
- In vivo D3 affects radioactive D3 in vitro
- 3[H]-1alpha,25(OH)2D3 in the intenstinal CMF
- VDR vs. DBP: which is present in the CMF?
- Colocalization of VDR and caveolin-1
- VDR knock out (KO) mouse
- 1,25D3 activity in WT vs. KO osteoblasts (1)
- 1,25D3 activity in WT vs. KO osteoblasts (2)
- Micrograph of WT and KO osteoblast
- [3H]-1alpha,25(OH)2D3 binding in KO mice
Topics Covered
- Optimal ligand shapes
- Modes of action of hormone-D
- Shape of ligand for VDRNUC
- Three dimensional shape of the VDR
- Shape of ligand for DBP
- DBP structure domains A, B, C
- Shape of ligand for rapid responses
- Steroid receptor superfamily
- Steroid receptor domains
- Structural organization of VDR gene, mRNA and protein
- Model of steroid hormone mode of action
- Model of hormone-D transcriptional activation
- Determination of a VDR mediated genomic response
- Examples of rapid responses
- Caveolae membrane and rapid responses
- VDR knock out mouse and rapid responses
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Talk Citation
Norman, A. (2007, December 1). Vitamin D endocrine system: mechanisms to produce biological responses [Video file]. In
The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved May 28, 2023, from
https://hstalks.com/bs/735/.
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Publication History
-
Published on December 1, 2007
Financial Disclosures
- Prof. Anthony Norman has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.