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1. Introduction
2. Mechanisms to produce biological responses
3. Optimal ligand shapes
4. Modes of action of 1alpha,25(OH)2D3
5. What are the different ligand shapes?
6. Shape of ligand for VDRnuc
7. Three dimensional shape of the VDR
8. 6-s-trans shape of the ligand for the VDR
9. Structural basis for binding to human DBP
10. DBP structure: domains A, B, C
11. Shape of the ligand for DBP
12. Shape of the ligand for rapid responses (1)
13. Shape of the ligand for rapid responses (2)
14. Summary of optimal ligand shapes
15. Genomic responses
16. Steroid receptor superfamily
17. Gene family of nuclear receptors for small ligands
18. Steroid receptor domains
19. Organization of VDR gene, mRNA and protein
20. Model of steroid hormone mode of action
21. Nuclear receptor zinc fingers
22. 1alpha,25(OH)2D3 transcriptional activation
23. Determination of VDR-mediated genomic response
24. Summary of genomic response
25. Rapid responses
26. Reminder: modes of action of 1alpha,25(OH)2D3
27. Examples of rapid responses
28. Molecular tools
29. Ion current potentiation by 1,25D3 in osteoblasts
30. Effect of 1beta,25(OH)2D3 on Cl-channels opening
31. Structure-function studies on 1alpha,25(OH)2D3
32. Structure-function analysis of rapid responses
33. Caveolae
34. Isolation of caveolae membrane fractions
35. [3H]-1alpha,25(OH)2D3 binding to CMF
36. Additional caveolae analyses
37. In vivo D3 affects radioactive D3 in vitro
38. 3[H]-1alpha,25(OH)2D3 in the intenstinal CMF
39. VDR vs. DBP: which is present in the CMF?
40. Colocalization of VDR and caveolin-1
41. VDR knock out (KO) mouse
42. 1,25D3 activity in WT vs. KO osteoblasts (1)
43. 1,25D3 activity in WT vs. KO osteoblasts (2)
44. Micrograph of WT and KO osteoblast
45. [3H]-1alpha,25(OH)2D3 binding in KO mice

Topics Covered

• Optimal ligand shapes
• Modes of action of hormone-D
• Shape of ligand for VDRNUC
• Three dimensional shape of the VDR
• Shape of ligand for DBP
• DBP structure domains A, B, C
• Shape of ligand for rapid responses
• Steroid receptor superfamily
• Steroid receptor domains
• Structural organization of VDR gene, mRNA and protein
• Model of steroid hormone mode of action
• Model of hormone-D transcriptional activation
• Determination of a VDR mediated genomic response
• Examples of rapid responses
• Caveolae membrane and rapid responses
• VDR knock out mouse and rapid responses

Links

Series:

• The World of Hormones

Categories:

• Biochemistry
• Metabolism & Nutrition
• Physiology & Anatomy

Therapeutic Areas:

• Cardiovascular & Metabolic

Talk Citation

Norman, A. (2007, December 1). Vitamin D endocrine system: mechanisms to produce biological responses [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved May 28, 2023, from https://hstalks.com/bs/735/.
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Publication History

• Published on December 1, 2007

Financial Disclosures

• Prof. Anthony Norman has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.