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- RNAi-Pathway Components and Mechanisms
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1. Activation of gene expression by double-stranded RNAs
- Prof. Long-Cheng Li
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2. MicroRNA biogenesis
- Dr. Narry Kim
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3. Dicer and R2D2
- Dr. Young Sik Lee
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4. Mammalian microRNA assembly and function
- Prof. Zissimos Mourelatos
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5. Mechanisms of RNAi: a molecular view or Argonaute: the secret life of Slicer
- Prof. Leemor Joshua-Tor
- Roles for RNAi in Development and Anti-Viral Defense
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6. Functional roles of RNAi in C. elegans
- Dr. Eric Miska
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7. RNAi and heterochromatin in plants and fission yeast
- Prof. Robert Martienssen
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8. RNA interference functions and mechanisms in animals
- Prof. Richard Carthew
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9. Specific functional roles in mammals
- Dr. Michael McManus
- RNAi Design Rules for Applications in Humans
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10. siRNA on and off-target
- Prof. Anastasia Khvorova
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11. Cell signalling pathways and RNAi
- Mr. Michael O'Grady
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12. RNAi: key technological aspects
- Dr. Dmitry Samarsky
- Dr. Ciaran Faherty
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13. RNAi specificity: how big of an issue is it?
- Dr. Peter Welch
- Applications of RNAi in the Treatment of Human Diseases
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14. siRNA therapeutics
- Prof. John Rossi
- Mr. Daniel J. Rossi
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15. RNAi for neurological diseases
- Prof. Beverly L. Davidson
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16. RNA interference and cancer: a revolution?
- Dr. Annick Harel-Bellan
- Archived Lectures *These may not cover the latest advances in the field
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17. RNAi and viral heart infections
- Dr. Jens Kurreck
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18. RNA interference for neurodegenerative diseases
- Prof. Beverly L. Davidson
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19. Activation of gene expression by double-stranded RNA
- Prof. David Corey
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20. New designs for siRNAs as Dicer substrate
- Dr. Mark Behlke
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21. RNAi in the model system Drosophila
- Prof. Richard Carthew
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23. Therapeutic delivery of RNAi
- Dr. Patrick Lu
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24. Mechanistic aspects and therapeutic applications of RNAi
- Prof. John Rossi
Printable Handouts
Navigable Slide Index
- Introduction
- Overview - RNA interference as an antiviral tool
- RNA interference pathway
- RNA interference methods
- Antisense vs. RNAi
- Inhibition of HIV-1 by RNAi
- Virus inhibition by RNAi - RNA viruses
- Virus inhibition by RNAi - DNA viruses
- Coxsackievirus B3 (CVB-3)
- Picornavirus replication
- Dilatated cardiomyopathy (DCM)
- Overview - design of efficient siRNAs
- siRNAs targeting the Coxsackievirus 5' UTR
- siRNAs against 5' UTR of CVB-3 have no effect
- Screening for efficient siRNAs against TRPV1
- What makes an efficient siRNA?
- Efficient siRNA - strand bias or target structure?
- Accessible nucleotides at 3' or 5' end of target site
- Activation of seemingly inactive siRNA
- Folding of artificial siRNA targets
- siRNA silencing and local stability - correlation
- Model for determinants of siRNA efficiency
- Overview - inhibition of coxsackievirus replication
- Targeting the viral RdRP
- Coxsackievirus replication in cell culture
- Cell viability assay with siRdRP 1-4
- Inhibition of virus replication by siRNA
- Inhibition of Coxsackievirus B3 by siRNA
- CVB-3 inhibition in vivo
- Overview - strand selectivity of virus silencing
- Replication cycle of picornaviruses
- Selective strand silencing
- Strand-selectivity of siRdRP
- Design of siRNAs targeting the RdRP (-)-strand
- Strand-specificity of silencing
- Strand specific siRNAs
- Strand-inactivation by incorporation of LNA
- siRNA vs. siLNA
- Overview - SiDEx
- Silencing of RdRP by shRNA expression plasmid
- Long-term silencing generates escape mutants
- Silencing of mutated RdRP
- SiRNA double expression vector (SiDEx)
- SiDEx silences two target sites
- SiDEx silences wt and mutated RdRP
- SiDEx - silencing of mutated RdRP
- Silencing of mutated target site 4
- Overview - silencing of CAR
- Coxsackievirus-adenovirus-receptor (CAR)
- Screening for efficient siRNAs targeting CAR
- Efficiency of CAR-siRNAs
- Duration of silencing
- Inhibition of virus propagation by CAR-siRNA
- SiDEx vector against two target sites of CVB-3
- Summary
- Acknowledgement
Topics Covered
- Coxsackievirus B3
- Design of efficient antiviral siRNAs
- Plaque reduction assays
- Virus escape
- siRNA double expression vectors (SiDEx)
- Strand selectivity of virus silencing
- Silencing of the host receptor (Coxsackievirus-adenovirus receptor)
Links
Series:
Categories:
Therapeutic Areas:
Talk Citation
Kurreck, J. (2007, October 1). RNAi and viral heart infections [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved March 29, 2024, from https://hstalks.com/bs/656/.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Jens Kurreck has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.