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- RNAi-Pathway Components and Mechanisms
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1. Activation of gene expression by double-stranded RNAs
- Prof. Long-Cheng Li
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2. MicroRNA biogenesis
- Dr. Narry Kim
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3. Dicer and R2D2
- Dr. Young Sik Lee
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4. Mammalian microRNA assembly and function
- Prof. Zissimos Mourelatos
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5. Mechanisms of RNAi: a molecular view or Argonaute: the secret life of Slicer
- Prof. Leemor Joshua-Tor
- Roles for RNAi in Development and Anti-Viral Defense
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6. Functional roles of RNAi in C. elegans
- Dr. Eric Miska
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7. RNAi and heterochromatin in plants and fission yeast
- Prof. Robert Martienssen
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8. RNA interference functions and mechanisms in animals
- Prof. Richard Carthew
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9. Specific functional roles in mammals
- Dr. Michael McManus
- RNAi Design Rules for Applications in Humans
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10. siRNA on and off-target
- Prof. Anastasia Khvorova
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11. Cell signalling pathways and RNAi
- Mr. Michael O'Grady
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12. RNAi: key technological aspects
- Dr. Dmitry Samarsky
- Dr. Ciaran Faherty
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13. RNAi specificity: how big of an issue is it?
- Dr. Peter Welch
- Applications of RNAi in the Treatment of Human Diseases
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14. siRNA therapeutics
- Prof. John Rossi
- Mr. Daniel J. Rossi
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15. RNAi for neurological diseases
- Prof. Beverly L. Davidson
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16. RNA interference and cancer: a revolution?
- Dr. Annick Harel-Bellan
- Archived Lectures *These may not cover the latest advances in the field
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17. RNAi and viral heart infections
- Dr. Jens Kurreck
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18. RNA interference for neurodegenerative diseases
- Prof. Beverly L. Davidson
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19. Activation of gene expression by double-stranded RNA
- Prof. David Corey
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20. New designs for siRNAs as Dicer substrate
- Dr. Mark Behlke
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21. RNAi in the model system Drosophila
- Prof. Richard Carthew
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23. Therapeutic delivery of RNAi
- Dr. Patrick Lu
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24. Mechanistic aspects and therapeutic applications of RNAi
- Prof. John Rossi
Printable Handouts
Navigable Slide Index
- Introduction
- RNA interference (RNAi)
- RNAi: Post-Transcriptional Gene Silencing (PTGS)
- RNAi: Transcriptional Gene Silencing (TGS)
- dsRNA mediated TGS in S. Pombe
- Do siRNAs directed to promoters induce TGS?
- FIV lentiviral vector system
- Experimental protocol
- siRNA targeting EF1a promoter suppress GFP
- Specificity of EF1A siRNA Mediated TGS
- siRNA does not alter vector integration
- siRNA mediated TGS is reversible
- TGS is associated with DNA methylation
- siRNA EF52 can target the EF1A promoter
- EF1a is only inhibited at 48hrs post-transfection
- Are intronic regions susceptible to siRNA targeting
- Intronic region is susceptible to siRNA targeting
- Specificity of EF315 transcriptional silencing
- siRNA mediated TGS in human cells
- What is the mechanism of siRNA mediated TGS?
- TGS operates via chromatin remodeling pathway
- siRNA mediated histone methylation
- RNA pol II is required for siRNA mediated TGS
- How do siRNAs find the target?
- Detection of flag-tagged DNMTs or Hp1s
- Specificity of DNMT binding
- Antisense siRNA targeting HIV-1 LTR/promoter
- Susceptibility of the HIV-1 LTR to siRNA
- siRNA EF52 and all the factors are linked
- Conclusions
- Putative mechanism(s) for siRNA mediated TGS
- Acknowledgements
- Living on the edge
Topics Covered
- How siRNAs mediate transcriptional gene silencing (TGS) in human cells
- Histone methylation
- Dependency on active RNA polymerase II mediated transcription
- siRNA-mediated TGS
- H3K9 and H3K27 methylation of the targeted EF1A promoter
- Nuclear specific delivery of the siRNA
- Antisense strand-specific siRNA-mediated TGS of EF1A
- Targeting of the U3 region of the HIV-1 LTR/promoter
- Antisense strand of siRNA EF2 interacts with FLAG-tagged DNMT3A and the tri-methylated H3K27 methyl mark
- siRNA mediated targeting of genomic regions
- RNA Pol-II function
- Transcriptional gene silencing in human cells
Talk Citation
Morris, K. (2007, October 1). Small interfering RNA induced transcriptional gene silencing in human cells [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved March 29, 2024, from https://hstalks.com/bs/651/.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Kevin Morris has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
Small interfering RNA induced transcriptional gene silencing in human cells
A selection of talks on Cell Biology
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