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- RNAi-Pathway Components and Mechanisms
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1. Activation of gene expression by double-stranded RNAs
- Prof. Long-Cheng Li
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2. MicroRNA biogenesis
- Dr. Narry Kim
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3. Dicer and R2D2
- Dr. Young Sik Lee
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4. Mammalian microRNA assembly and function
- Prof. Zissimos Mourelatos
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5. Mechanisms of RNAi: a molecular view or Argonaute: the secret life of Slicer
- Prof. Leemor Joshua-Tor
- Roles for RNAi in Development and Anti-Viral Defense
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6. Functional roles of RNAi in C. elegans
- Dr. Eric Miska
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7. RNAi and heterochromatin in plants and fission yeast
- Prof. Robert Martienssen
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8. RNA interference functions and mechanisms in animals
- Prof. Richard Carthew
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9. Specific functional roles in mammals
- Dr. Michael McManus
- RNAi Design Rules for Applications in Humans
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10. siRNA on and off-target
- Prof. Anastasia Khvorova
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11. Cell signalling pathways and RNAi
- Mr. Michael O'Grady
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12. RNAi: key technological aspects
- Dr. Dmitry Samarsky
- Dr. Ciaran Faherty
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13. RNAi specificity: how big of an issue is it?
- Dr. Peter Welch
- Applications of RNAi in the Treatment of Human Diseases
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14. siRNA therapeutics
- Prof. John Rossi
- Mr. Daniel J. Rossi
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15. RNAi for neurological diseases
- Prof. Beverly L. Davidson
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16. RNA interference and cancer: a revolution?
- Dr. Annick Harel-Bellan
- Archived Lectures *These may not cover the latest advances in the field
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17. RNAi and viral heart infections
- Dr. Jens Kurreck
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18. RNA interference for neurodegenerative diseases
- Prof. Beverly L. Davidson
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19. Activation of gene expression by double-stranded RNA
- Prof. David Corey
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20. New designs for siRNAs as Dicer substrate
- Dr. Mark Behlke
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21. RNAi in the model system Drosophila
- Prof. Richard Carthew
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23. Therapeutic delivery of RNAi
- Dr. Patrick Lu
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24. Mechanistic aspects and therapeutic applications of RNAi
- Prof. John Rossi
Printable Handouts
Navigable Slide Index
- Introduction
- The pathway of gene expression
- Regulatory roles for RNA
- Defining the RNAi mechanism
- The discovery of regulatory RNA
- RNAi
- dsRNA, siRNA and miRNA
- Conversion of dsRNA to siRNA
- Extracts from Drosophila cells and Dicer activity
- The Drosophila Dicer family
- Dcr-2 is essential for siRNA production
- The initiation and effector phases
- Biochemical definition of Drosophila RISC
- RISC-associated proteins
- Dcr-2 and R2D2
- siRNAs fail to program RISC if Dcr-2 is missing (1)
- siRNAs fail to program RISC if Dcr-2 is missing (2)
- Native gel electrophoresis
- Examples
- Role of Dicers in RISC (1)
- Role of Dicers in RISC (2)
- mRNA become targets for destruction
- The biological functions of RNAi
- MicroRNAs in Drosophila
- Polycistronic and monocistronic miRNA genes
- Mechanism of gene silencing by miRNAs
- Differential Gel Electrophoresis - DIGE
- DIGE analysis of the oocyte proteome
- The detectable proteome is repressed by miRNAs
- miRNAs mute rather than silence most targets
- miRNAs repress protein synthesis from mRNAs
- Expression of genes involved in protein metabolism
- Genes silenced by miRNAs
- The biological function of miRNAs
- The Drosophila ovary: stem cells in action
- Dcr-1 mutant germ line interrupting development
- Dcr-1 promotes division rate of germ stem cells
- Dcr-1 promotes G1/S transition in germ stem cells
- Dcr-1 represses p21/Dap expression
- p21/Dap is downstream of Dcr-1 in G1/S pathway
- Dcr-1 is not essential for division in somatic cells
- miRNAs and stem cell growth:a model
- Conclusions
- Thanks
Topics Covered
- Understanding how RNAi works
- The fruitfly, Drosophila melanogaster model
- The three reasons for the success of the model
- RNAi mechanism in Drosophila and humans is similar
- Rich history of genetics in Drosophila
- Robust chemistry of the Drosophila system
- Fusion of genetics and biochemistry
- Discovery of RISC
- Discovery of Dicer
- Discovery of Ago2
- New features of RNAi biochemistry
- Greater understanding of how double-helix RNA is used to silence genes
- Development of RNAi for disease research and treatment
Talk Citation
Carthew, R. (2007, October 1). RNAi in the model system Drosophila [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 20, 2024, from https://hstalks.com/bs/645/.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Richard Carthew has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.