In this talk, we'll be going over the characteristics of the self-splicing intron RNAs,
which include the group one and the group two introns,
and hopefully by the end,
you'll see what kinds of properties they have as ribozymes, as folded RNAs,
and see what kind of context they have biologically
as parasites and agents of genomic change.
The latter part of the talk will
include experiments that were done primarily in my own lab,
and your narrator is indicated by the yellow arrow in this picture.
But it's important to say that much of the talk will also be about group
one introns and I worked on those as a postdoc in Tom Cech's lab.
Before we review the specifics of group one and group two introns,
it's important to review what an intron actually is.
When a gene is actually transcribed into RNA,
there are regions of the RNA that contain information
that is not supposed to make its way into the functional product.
And these intervening regions of sequence,
which I've shown here in blue are called introns.
The functional regions of the RNA are called exons,
shown here as exon 1 and exon 2.
Through the process of splicing,
introns are removed and discarded and
the exons are stitched together again to create a functional gene.
It's important to point out that a typical gene,
encoding one of your proteins, i.e.
a eukaryotic gene, is highly complex and requires
the splicing of many exons and removal of many introns.
So splicing is a fundamental process in gene expression, particularly in eukaryotes.