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Printable Handouts
Navigable Slide Index
- Introduction
- The nucleosome
- Histone post-translational modifications
- Histones during transcriptional activation
- Site specific recombination at the POH5 locus
- Linking difference due to core particles removal
- Chromatin remodeling
- Measurment of relative nucleosome number
- Nucleosomes retained on activated promotor
- Nucleosomes are retained with differet probabilities
- Results of limit nuclease and topology analysis
- Why fractional value?
- Two possible mechanisms of nucleosome removal
- Reversing the order of activation and recombination
- Results of the order reversing
- Nucleosome removal: experimnetal results
- Nucleosome removal: conclusions
- Histone octamer transfer by the RSC
- Assembly of transcription machinery
- RNA polymerase II underlines the central dogma
- RNA pol-II transcription machinery
- Regulation of RNA pol II transcription
- Conserved Mediator subunits
- Mediator of transcriptional regulation
- Structure of RNA pol II transcription machinery
- 2-D protein crystallization
- Obstacles to x-ray structure determination of pol II
- Data on structure of pol II
- Location of atoms of pol II
- Polypeptide chains of pol II
- Cleft of pol II
- The nucleic acids in the transcription complex
- The clamp
- Structure of transcribing complex
- Zoom-in on active center
- Nucleotide addition cycle
- Bridge helix structure
- Surface representaion of the entire pol II
- Upstream end of the DNA-RNA hybrid
- Fork loops 1, rudder and lid
- Transcriptions proteins interactions
- Pol-II - TFIIB complex: N-termianl domain
- TFIIB finger domain penetrates the cleft
- B-finger and DNA-RNA hybrid
- TFIIB stabilizes an initial transcribing complex
- TFIIB-transcript competition
- Pol-II-TFIIB complex: C-terminal domain
- Structure of C-terminal domain of TFIIB
- TBP structure
- DNA bending along pol II surface
- DNA bending in surface representation
- Complete transcriptional initiation complex
- Mechanism of initiation of pol II transcription
- Modular structure of mediator
- Conclusions
Topics Covered
- Chromatin remodeling for transcription
- The RNA polymerase II transcription machinery
- Mediator of transcriptional regulation
- Atomic structure of RNA polymerase II and of a transcribing complex
- Structural basis of ribonucleotide addition and transcript elongation
- Structure of a complete pre-initiation complex
Talk Citation
Kornberg, R. (2007, October 1). Chromatin and transcription [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 5, 2024, from https://doi.org/10.69645/SOIL2711.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Roger Kornberg has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.