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- View the Talks
- Gene drive systems
-
2. Different types of gene drives
- Prof. Jackson Champer
-
3. Population modification of malaria vector mosquitoes
- Dr. Anthony A. James
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5. CRISPR-based suppression drives for vector control
- Prof. Andrea Crisanti
- Active genetics and drive effector factors
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8. The dawn of active genetics
- Prof. Ethan Bier
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10. Ecological considerations for gene drive systems
- Prof. Gregory C. Lanzaro
- Mathematical modeling
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12. Gene drive behavior when pest populations have age, mating and spatial structure
- Prof. Fred Gould
- Prof. Alun Lloyd
- Social and ethical considerations
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13. The risks and benefits of gene drive technology
- Prof. Henry Greely
-
14. Guidance for responsible testing and implementation of gene-drive systems
- Prof. Stephanie James
-
16. CRISPR editing therapy for Duchenne Muscular Dystrophy 1
- Prof. Dongsheng Duan
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17. CRISPR editing therapy for Duchenne Muscular Dystrophy 2
- Prof. Dongsheng Duan
Printable Handouts
Navigable Slide Index
- Introduction
- Talk outline
- Medea
- Medea drive experiments
- Public attitude surveys in Mali
- The Cartagena protocol & GM mosquitoes
- A confined trial of a gene drive system
- Semele
- The naming of Semele
- Introduction of Semele
- UDMEL
- Inheritance pattern of UDMEL
- UDMEL drive experiments
- Introduction of UDMEL
- Translocations display threshold dynamics
- Translocation drive experiments
- Fine-scale landscape genomics
- The An. gambiae life cycle
- MGDrivE modeling framework
- MGDrivE: mosquito ecology module
- MGDrivE: translocations with remediation
- MGDrivE: UDMEL without remediation
- MASH modeling framework
- MASH: mosquito movement patterns
- Spread of a gene drive system
- Homing-based gene drive systems
- Homing gene drive systems
- Confineability of homing gene drive systems
- Targeting a female fertility gene
- Achieving population elimination
- Multiplexing gRNAs
- Conclusion
- Acknowledgements
Topics Covered
- Mathematical Modeling of Gene Drive
- Medea
- Toxin-antidote-based underdominant systems
- Reciprocal chromosomal translocations
- Spatially-structured modeling frameworks
- Confineable homing-based gene drive systems
- Potential for invasive homing-based systems following field trials
Talk Citation
Marshall, J. (2018, July 31). Gene drive: what is possible at the population level with currently-available molecular components? [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 4, 2024, from https://doi.org/10.69645/YJMM9765.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. John Marshall has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
Gene drive: what is possible at the population level with currently-available molecular components?
Published on July 31, 2018
52 min
A selection of talks on Methods
Transcript
Please wait while the transcript is being prepared...
0:00
I'm John Marshall, an Assistant Professor in Residence at the University of California,
Berkeley in the School of Public Health.
And I'm going to be giving a talk on Gene Drives and Active Genetics.
Specifically on gene drive,
what is possible at the population level with currently available molecular components?
0:21
So, the work that I do focuses on mathematical modeling of gene drive systems.
So, I'm going to be talking about them from that perspective.
And to begin with, we will talk about Medea and underdominant drive systems.
So, Medea itself, is toxin-antidote-based underdominant systems including Semele and UDMEL,
and also reciprocal chromosomal translocations.
So then, we'll have to talk about
the mathematical modeling frameworks that can be used to study
the spread of these gene drive systems through spatially-structured mosquito populations.
One of which is MGDrivE,
which stands for Mosquito Gene Drive Explorer
and another is MASH or Modular Analysis and Simulation for human Health.
And then, we will discuss homing-based gene drive systems and the potentials developed
confineable homing-based systems that may be able
to be limited and extend to their spread spatially,
and also the potential for invasive homing-based gene drive systems
following field trials when it may be desired,
they spread on a wider scale.
1:34
Beginning with Medea, Medea was the first synthetic gene drive system to be engineered.
It was engineered in the lab of Bruce Hay at Caltech,
which I was a postdoc in, and it's based on the function of
the maternal toxin which is linked to a zygotic antidote.
So, if we look at the crosses,
then heterozygous or homozygous mothers
produce a toxin which will affect all of their offspring.
And then the offspring, if they inherit the zygotic antidote
as part of the Medea element, will be protected against the action of this toxin,
and the results in the rightmost cross is that
only wild-type offspring of heterozygous females
will be rendered unviable by this construct.
But the result of that is that it actually places a selective advantage
on having the Medea construct and a disadvantage on not having it,
and therefore, over time,
the Medea elements spreads into the population and is
capable of spreading to the population from very low initial frequencies.
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