Printable Handouts

PDF

Navigable Slide Index

1. Introduction
2. Protein folding and aggregation
3. Thermotolerance
4. Protein synthesis during heat shock
5. Hsp104 is crucial for thermotolerance in yeast
6. Hsp104 restores ultrastructure after heat shock
7. Hsp104-dependent resolubilization of proteins
8. Hsp104-dependent enzyme activity reactivation
9. Hsp104 has unique properties
10. The assay for Hsp104-dependent refolding
11. Hsp104 works together with Hsp70/40
12. Hsp104 is required for the refolding of proteins
13. Bichaperone networks
14. The second aggregation mode
15. Prions
16. Prions in yeast
17. The prion form of Sup35 is aggregated
18. Prions recruit newly synthesized Sup35
19. Self-seeded aggregation of Sup35 in vitro
20. Nonsense codon suppression in [PSI+] cells
21. Hsp104 and prion stability
22. Prion aggregate disassembly
23. Hsp104 and Sup35 fibril assembly
24. Hsp104: a member of AAA+ superfamily
25. AAA+ complexes
26. The Clp/Hsp100 family
27. Clp/Hsp100 complexes
28. A structural model of ClpB
29. Hsp100-mediated degradation
30. Axial channel loops
31. Mutations in axial loops reduce thermotolerance
32. Common mechanism of Hsp100 function?
33. Aggregates may be dynamic structures
34. Partial disaggregation by molecular chaperones
35. Hsp104 completes the release of proteins
36. Chaperones bind disease-associated proteins
37. Hsp104 functions in hybrid bichaperone network
38. Hsp104 transgene reduces aggregation
39. Future questions

Topics Covered

• Heat shock
• Molecular chaperones and thermotolerance
• Protein aggregation and refolding
• Prions in mammals and yeast
• Molecular chaperones and protein aggregation associated with human disease

Links

Series:

• Molecular Chaperones

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Glover, J.R. (2007, October 1). Hsp104: a specialized chaperone for protein disaggregation [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 20, 2024, from https://hstalks.com/bs/344/.
Export Citation (RIS)

Publication History

• Published on October 1, 2007
• Archived on February 25, 2021

Financial Disclosures

• Dr. John R. Glover has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.