We noted you are experiencing viewing problems
-
Check with your IT department that JWPlatform, JWPlayer and Amazon AWS & CloudFront are not being blocked by your network. The relevant domains are *.jwplatform.com, *.jwpsrv.com, *.jwpcdn.com, jwpltx.com, jwpsrv.a.ssl.fastly.net, *.amazonaws.com and *.cloudfront.net. The relevant ports are 80 and 443.
-
Check the following talk links to see which ones work correctly:
Auto Mode
HTTP Progressive Download Send us your results from the above test links at access@hstalks.com and we will contact you with further advice on troubleshooting your viewing problems. -
No luck yet? More tips for troubleshooting viewing issues
-
Contact HST Support access@hstalks.com
-
Please review our troubleshooting guide for tips and advice on resolving your viewing problems.
-
For additional help, please don't hesitate to contact HST support access@hstalks.com
We hope you have enjoyed this limited-length demo
This is a limited length demo talk; you may
login or
review methods of
obtaining more access.
Printable Handouts
Navigable Slide Index
- Introduction
- Origins and diversity of the basic animal body plan
- Ernst Haeckel, Anthropogenie
- Ernst Haeckel “Tree of life”
- Most vertebrate embryos look the same
- How does diversity follow from similarity?
- Hox homeotic genes
- A common origin
- Vertebrate Hox genes co-linearity and expression
- Combinatorial “Hox code”
- Conservation of Hox genes in vertebrates
- Common genes in different organisms
- The phylotypic stage
- Hox genes and axial identity in development
- The vertebrate hindbrain and Hox genes
- Patterning brain and facial development
- Segmental organization of the hindbrain
- Conservation of hindbrain segmentation
- Organization of the hindbrain
- Alternating segmental properties and segmentation
- Transcriptional profiling individual rhombomeres
- Segmental patterns of gene expression
- Monitoring nested Hox expression
- Segmental organization of the hindbrain
- Hox genes regulate segmental identity
- Hox genes regulate multiple steps of segmentation
- The nervous system and craniofacial development
- Formation and fate of neural crest cells
- Hindbrain segmentation and neural crest migration
- Neural crest cells migration and differentioation
- Segmental organization of the hindbrain
- How are AP patterns of Hox expression set up?
- Hox genes and axial identity specification
- Analysis of cis-regulation of HoxB genes
- Segmental hindbrain HoxB expression regulation
- Modular cis-regulatory control of Hoxa2
- Hindbrain cis-regulatory modules of Hox genes
- Vertebrate hindbrain segmentation GRN
- Regulation of vertebrate hindbrain organisation
- Common role for auto/cross-regulation
- Previously characterized Hox/Pbx binding sites
- The rhombomere 4 regulatory network
- DV patterns of Hox expression
- Segmental and neuronal functions of Hox genes
- Complex cis-regultory landscape
- Hox genes as morphogenic information integrators
- Opposing signaling centers drive axial extension
- Regulators of Hox gene expression
- Hox expression regulation and retinoids
- Where are the RAREs and how do they function?
- HoxB multiplex reporter BAC
- RAREs are necessary for expansion of 5’ Hoxb
- Summary: Hox gene regulation by multiple RAREs
- Conserved and unique RAREs in Hox clusters
- Is DE-RARE is responsible for anterior expansion?
- DE-RARE can anteriorize 5’ Hoxa gene expression
- Hox genes, retinoids and heart development
- Retinoids and Hox genes in heart development
- Deletion of the HoxA and HoxB complexes
- Where are the heart regulatory regions
- Identification of novel HoxB control regions
- Novel RAREs in heart and endoderm enhancers
- Identification of novel HoxB control regions
- Cooperative/shared/selective/long-range enhancers
- Building the gene regulatory network
- Acknowledgements
- Stowers Institute
Topics Covered
- Origins and diversity of the basic animal body plan
- Ernst Haeckel: Anthropogenie, “Tree of life”
- Common mechanisms for vertebrate body plan
- How does diversity follow from similarity?
- Hox homeotic genes are clustered and control head to tail patterning
- Vertebrate Hox genes display co-linear and segmental expression
- “Hox code”
- Conservation of Hox genes in vertebrates
- The phylotypic stage
- The vertebrate hindbrain, head development & Hox genes: A story in segments
- The nervous system plays an important role in regulating craniofacial development through formation of neural crest cells
- Roles for hindbrain segmentation & neural crest migration in craniofacial patterning
- How are the AP patterns of Hox expression set up?
- Analysis of cis-regulation of HoxB genes
- Modular cis-regulatory control of Hoxa2
- Vertebrate hindbrain segmentation GRN
- Regulation of vertebrate hindbrain organisation
- Previously characterized consensus Hox/Pbx binding sites
- The rhombomere 4 regulatory network
- DV patterns of Hox expression
- Segmental and neuronal functions of Hox genes
- Complex cis-regultory landscape controls Hox gene expression in the specification of axial identity
- Hox genes as integrators of morphogenic information
- Opposing signaling centers drive axial extension: Wnt and Fgf signaling oppose RA signaling
- Regulators of Hox gene expression
- What mechanisms do retinoids use to regulate Hox expression?
- Where are the RAREs and how do they function in the Hox clusters?
- HoxB multiplex reporter BAC reproduces endogenous gene expression pattern
- RAREs are necessary for anterior expansion of 5’ Hoxb expression in the neural tube
- Conserved and unique RAREs in Hox clusters
- Is DE-RARE responsible for the anterior expansion?
- DE-RARE can anteriorize 5’ Hoxa gene expression
- Hox genes, retinoids and heart development
- Where are the heart regulatory regions
- Identification of novel HoxB control regions
- Novel RAREs in heart & endoderm enhancers
- Cooperative, shared, selective and long-range enhancers
- Building the gene regulatory network which governs segmental, neuronal and patterning functions of Hox genes
Links
Series:
Categories:
Talk Citation
Krumlauf, R. (2014, September 3). Hox gene regulation in vertebrate hindbrain development [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 9, 2024, from https://doi.org/10.69645/KSLZ2094.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Robb Krumlauf has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
A selection of talks on Biochemistry
Transcript
Please wait while the transcript is being prepared...
0:00
Hello, my
name is Robb Krumlauf,
and I am the scientific
director of the Stowers
Institute for Medical Research.
What I'd like to talk to you
about today in this lecture
are discussion of the
Hox gene regulation
in vertebrate hindbrain development.
0:18
To begin our lecture, I would
like to discuss by introduction
the biological question
that we're interested in.
The title of this slide
is origins and diversity
of the basic animal body plan.
In this beautiful
artist's interpretation,
you can see that the
body plans of animals
are very different from each other.
And throughout history
we have wondered
how these plans are laid down.
Are plans specific for a mouse,
or a human, or other organisms?
What we are beginning
to realize is that there
are common roots to
all of these plans.
And that is going to be a
theme that I talk to you
about through understanding
the function of Hox genes.
1:02
It is interesting that
historically embryologists
have been aware of this
problem for a long time.
In this cover sheet from a famous
book by Ernst Haeckel published
in 1874, he discusses interesting
features of the developing embryos.
1:22
In his book, this is a beautiful
representation of the first example
of what many refer to
as a phylogenetic tree.
It shows the branches
and roots for how
animals are related to each other.
And this work now has
become very advanced,
but it was very thought
provoking at the time.