• Introduction to Calcium Signaling
• 72 min
  1. Introduction to cellular calcium signaling

  • Dr. Martin Bootman
• 20 min
  2. Monitoring Ca2+ concentration in living cells

  • Dr. Marisa Brini
• 30 min
  3. Cell boundary theorem

  • Prof. Eduardo Ríos
• Calcium Influx
• 19 min
  4. Arachidonic acid and store-independent Ca2+ entry

  • Dr. Luca Munaron
• 34 min
  5. Voltage-dependent calcium channels

  • Prof. Annette Dolphin
• 34 min
  6. Protein networks of voltage-gated Ca2+ channels – proteomic & functional analysis of local Ca2+ signalling

  • Prof. Bernd Fakler
• 37 min
  7. Intracellular Ca2+ signaling: calcium influx

  • Prof. Anant Parekh
• 60 min
  8. Molecular identification of the CRAC channel

  • Prof. Michael Cahalan
• 35 min
  9. TRP channels with diverse permeability profiles: regulation of blood pressure and fertility

  • Prof. Marc Freichel
• Calcium Release
• 47 min
  10. The molecular biology of the inositol trisphosphate receptor

  • Dr. Randen Patterson
• 53 min
  11. The InsP3 receptor calcium release channel

  • Prof. J. Kevin Foskett
• 31 min
  12. Molecular biology of ryanodine receptors

  • Dr. Christopher George
  • Prof. F. Anthony Lai
• 57 min
  13. cADPR and NAADP: messengers for calcium signalling

  • Prof. Antony Galione
• 50 min
  14. Coordinated Ca2+ release from intracellular Ca2+ stores

  • Prof. Ole Petersen
• 43 min
  15. Ryanodine receptors and cardiac function

  • Prof. David Eisner
• Calcium Efflux and Sequestration
• 45 min
  16. The plasma membrane calcium pump: biochemistry, physiology and molecular pathology

  • Prof. Ernesto Carafoli
• 60 min
  17. The calcium saga: a matter of life and death

  • Prof. Pierluigi Nicotera
• 45 min
  18. Sodium-calcium exchange

  • Prof. John Reeves
• 22 min
  19. Ca2+ efflux and Ca2+ signals

  • Dr. Anne Green
• Organelle Calcium
• 42 min
  20. Regulation and role of mitochondrial Ca2+ homeostasis

  • Prof. Rosario Rizzuto
• 54 min
  21. Peroxisomes and Golgi apparatus as players in Ca2+ homeostasis

  • Dr. Paola Pizzo
  • Dr. Alex Costa
• 31 min
  22. Organellar calcium pumps of the golgi and ER: from yeast to human

  • Prof. Rajini Rao
• 34 min
  23. Ca2+ dynamics between mitochondria and the endoplasmic reticulum

  • Dr. Wolfgang Graier
• 26 min
  24. Nuclear calcium signaling

  • Dr. Oleg Gerasimenko
  • Dr. Julia Gerasimenko
• Spatiotemporal Calcium Signals
• 58 min
  25. Spatial and temporal aspects of cellular calcium signals: 'elementary' signals and waves

  • Prof. Ian Parker
• 54 min
  26. Regulation of intracellular calcium signaling, localized signals and oscillations

  • Prof. Barbara Ehrlich
• 26 min
  27. Modeling Ca2+ signals

  • Dr. David Friel
• Calcium Effectors
• 34 min
  28. Neuronal calcium sensor proteins: calcium sensors and regulators of diverse cellular functions

  • Prof. Bob Burgoyne
• 41 min
  29. Calcium-regulated adenylyl cyclases and cyclic AMP compartmentalization

  • Prof. Dermot Cooper
• 32 min
  30. Calcium and transcription-coupling

  • Dr. Karen Lounsbury
• 50 min
  31. Cellular calcium (Ca2+) buffers

  • Prof. Dr. Beat Schwaller
• 24 min
  32. Extracellular calcium signaling

  • Dr. Aldebaran M. Hofer
• 50 min
  33. Ca2+, fertilization and egg activation

  • Prof. Karl Swann
• 24 min
  34. Calcium regulation of transcription in plants

  • Prof. Hillel Fromm
• 34 min
  35. Mechanisms regulating STIM expression and function in Ca2+ signaling

  • Dr. Jonathan Soboloff
• 46 min
  36. Dynamic signal encoding in the S. cerevisiae calcium response

  • Dr. Chiraj Dalal
• Calcium and Disease
• 43 min
  37. Polycystins, calcium signaling and pathogenesis of polycystic kidney disease

  • Prof. Laura del Senno
• 28 min
  38. Ca2+ alterations in familial Alzheimer's disease (FAD)

  • Dr. Paola Pizzo
• 38 min
  39. Pancreatitis and calcium signaling

  • Prof. Ole Petersen
• 47 min
  40. Mechanism-based therapies for heart failure and cardiac arrhythmias

  • Prof. Andrew Marks
• 46 min
  41. Genetic defects and calcium

  • Prof. Tullio Pozzan
• Archived Lectures *These may not cover the latest advances in the field
• 31 min
  42. Calcium, calmodulin and calcineurin

  • Prof. Stephen Bolsover
• 34 min
  43. Calcium flickers steer cell migration

  • Prof. Heping Cheng
• 28 min
  44. Automated Ca2+ imaging of chemosensory neurones in C.elegans

  • Dr. Nikos Chronis
• 46 min
  45. Ca2+ and the regulation of small GTPases

  • Prof. Peter Cullen
• 35 min
  46. Genetically encoded Ca2+ indicators: molecular scale measurements in mammals in vivo

  • Dr. Michael I. Kotlikoff
• 35 min
  47. Capacitative (store-operated) calcium entry

  • Dr. Jim Putney

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Part 1 - peroxisomes
3. Peroxisomes (continued)
4. Peroxisomal Ca2+ homeostasis
5. Methods for peroxisomal Ca2+ measurements
6. Aequorin method
7. Cameleon method
8. GFP-based Ca2+ probe: cameleon (1)
9. GFP-based Ca2+ probe: cameleon (2)
10. GFP-based Ca2+ probe: cameleon (3)
11. Cameleon expression in HeLa and GH3 cells
12. Cameleon expression in the peroxisomal lumen
13. Peroxisome Ca2+ increases in GH3 cells
14. Ca2+ rise in HeLa and not in GH3 cells
15. Effects of ATP, H+, or Na+ on Ca2+ uptake
16. In situ calibration of cameleon fluorescence
17. Ca2+ in cytosol and peroxisome lumen
18. Conclusions (1)
19. Plant peroxisomes overview
20. A functional role of Ca2+ in plant peroxisomes (1)
21. A functional role of Ca2+ in plant peroxisomes (2)
22. A functional role of Ca2+ in plant peroxisomes (3)
23. Cameleon in epidermal cells of tobacco leaves
24. Cameleon for analysis of Ca2+ dynamics
25. Cameleon expression in transgenic Arabidopsis
26. Analyses at organ/tissue level
27. Transient Ca2+ in arabidopsis root cells (1)
28. Transient Ca2+ in arabidopsis root cells (2)
29. Single cell analyses
30. Cytoplasmic and peroxisomal Ca2+ in guard cells
31. In vivo intraperoxisomal imaging in guard cells
32. Ca2+-dependent peroxisomal H2O2 scavenging
33. Peroxisomal H2O2 scavenging efficiency
34. Conclusions (2)
35. References - part 1
36. Part 2 - the Golgi apparatus
37. The Golgi apparatus: overview
38. The Golgi as an intracellular Ca2+ store (1)
39. The Golgi as an intracellular Ca2+ store (2)
40. The Golgi as a rapidly mobilizable Ca2+ store (1)
41. The Golgi as a rapidly mobilizable Ca2+ store (2)
42. The Golgi as a rapidly mobilizable Ca2+ store (3)
43. The Golgi is heterogeneous in Ca2+ handling
44. A cameleon Ca2+ sensor for the trans-Golgi
45. Cameleon Ca2+ sensor is localized in trans-Golgi
46. Ca2+ handling by trans-Golgi in intact cells
47. Ca2+ uptake in trans-Golgi is mediated by SPCA1
48. Ryanodine receptor decreases Ca2+ in trans-Golgi
49. Ca2+ concentration within the trans-Golgi
50. Cameleon Ca2+ sensor for the medial-Golgi
51. A new cameleon Ca2+ sensor for the medial-Golgi
52. Ca2+ handling by medial-Golgi in intact cells
53. Medial-Golgi Ca2+ uptake by SERCA and SPCA1
54. Ryanodine receptor and [Ca2+] in medial-Golgi
55. Ca2+ concentration within the medial-Golgi
56. Free Ca2+ concentration gradient
57. What is the functional role of luminal Golgi Ca2+?
58. Effects of SPCA1 down-regulation
59. SPCA1 knock-down EM
60. Decreased SPCA1 and Golgi Ca2+ and disease
61. The Golgi apparatus - conclusions
62. Part 2: the Golgi apparatus - references
63. Acknowledgments

Topics Covered

• Role of Peroxisomes and Golgi apparatus within the cell
• Methodologies to study peroxisomal and Golgi apparatus Ca2+ signals
• Ca2+ handling by mammalian and plant peroxisomes and its functional role
• Differential Ca2+ homeostasis in sub-regions of the Golgi apparatus and functional roles

Links

Series:

• Calcium Signaling I

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Publication History

• Published on November 5, 2013
• Reviewed on November 22, 2019

Financial Disclosures

• Dr. Paola Pizzo has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
• Dr. Alex Costa has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.