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Printable Handouts
Navigable Slide Index
- Introduction
- Part 1 - peroxisomes
- Peroxisomes (continued)
- Peroxisomal Ca2+ homeostasis
- Methods for peroxisomal Ca2+ measurements
- Aequorin method
- Cameleon method
- GFP-based Ca2+ probe: cameleon (1)
- GFP-based Ca2+ probe: cameleon (2)
- GFP-based Ca2+ probe: cameleon (3)
- Cameleon expression in HeLa and GH3 cells
- Cameleon expression in the peroxisomal lumen
- Peroxisome Ca2+ increases in GH3 cells
- Ca2+ rise in HeLa and not in GH3 cells
- Effects of ATP, H+, or Na+ on Ca2+ uptake
- In situ calibration of cameleon fluorescence
- Ca2+ in cytosol and peroxisome lumen
- Conclusions (1)
- Plant peroxisomes overview
- A functional role of Ca2+ in plant peroxisomes (1)
- A functional role of Ca2+ in plant peroxisomes (2)
- A functional role of Ca2+ in plant peroxisomes (3)
- Cameleon in epidermal cells of tobacco leaves
- Cameleon for analysis of Ca2+ dynamics
- Cameleon expression in transgenic Arabidopsis
- Analyses at organ/tissue level
- Transient Ca2+ in arabidopsis root cells (1)
- Transient Ca2+ in arabidopsis root cells (2)
- Single cell analyses
- Cytoplasmic and peroxisomal Ca2+ in guard cells
- In vivo intraperoxisomal imaging in guard cells
- Ca2+-dependent peroxisomal H2O2 scavenging
- Peroxisomal H2O2 scavenging efficiency
- Conclusions (2)
- References - part 1
- Part 2 - the Golgi apparatus
- The Golgi apparatus: overview
- The Golgi as an intracellular Ca2+ store (1)
- The Golgi as an intracellular Ca2+ store (2)
- The Golgi as a rapidly mobilizable Ca2+ store (1)
- The Golgi as a rapidly mobilizable Ca2+ store (2)
- The Golgi as a rapidly mobilizable Ca2+ store (3)
- The Golgi is heterogeneous in Ca2+ handling
- A cameleon Ca2+ sensor for the trans-Golgi
- Cameleon Ca2+ sensor is localized in trans-Golgi
- Ca2+ handling by trans-Golgi in intact cells
- Ca2+ uptake in trans-Golgi is mediated by SPCA1
- Ryanodine receptor decreases Ca2+ in trans-Golgi
- Ca2+ concentration within the trans-Golgi
- Cameleon Ca2+ sensor for the medial-Golgi
- A new cameleon Ca2+ sensor for the medial-Golgi
- Ca2+ handling by medial-Golgi in intact cells
- Medial-Golgi Ca2+ uptake by SERCA and SPCA1
- Ryanodine receptor and [Ca2+] in medial-Golgi
- Ca2+ concentration within the medial-Golgi
- Free Ca2+ concentration gradient
- What is the functional role of luminal Golgi Ca2+?
- Effects of SPCA1 down-regulation
- SPCA1 knock-down EM
- Decreased SPCA1 and Golgi Ca2+ and disease
- The Golgi apparatus - conclusions
- Part 2: the Golgi apparatus - references
- Acknowledgments
Topics Covered
- Role of Peroxisomes and Golgi apparatus within the cell
- Methodologies to study peroxisomal and Golgi apparatus Ca2+ signals
- Ca2+ handling by mammalian and plant peroxisomes and its functional role
- Differential Ca2+ homeostasis in sub-regions of the Golgi apparatus and functional roles
Talk Citation
Pizzo, P. and Costa, A. (2019, November 22). Peroxisomes and Golgi apparatus as players in Ca2+ homeostasis [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 7, 2024, from https://doi.org/10.69645/JCLU4520.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Paola Pizzo has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
- Dr. Alex Costa has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
A selection of talks on Biochemistry
Transcript
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0:00
Hello.
My name is Alex Costa.
I am a plant biologist and
I work at University of Milan in the
Department of Biosciences, together with
Paola Pizzo from University of Padua
department of biomedical sciences.
We will talk about peroxisomes and
golgi apparatus as players
in calcium homeostasis.
0:22
Peroxisomes are organelles present
in almost all eukaryotic cells.
They are enclosed by a single
lipid bilayer membrane and
inside the peroxisomal lumen,
more than 50 enzymes have been discovered.
This enzymes play roles in different
metabolic pathways, including fatty acid,
beta oxidation, scavenging of hydrogen
peroxide, synthesis of etherphospholipids.
Peroxisomes are numerous and
dispersed throughout the entire cytosol.
And their size and
number is regulated by a specific
transcription factor in mammalian cells.
Peroxisomal membrane is impermeable to
high molecular weight molecules, and for
this reason, specific carriers
are expressed in the organelles for
taking up different metabolites.
1:20
Peroxisome activity has been linked
with many different processes.
For example,
the peroxisomes have emerged as organelles
important in determining cell fate.
The activity has been related to
cell differentiation processes,
embryo development and morphogenesis.
Moreover, impairment of peroxisomal
activity or biogenesis is linked
to different genetic disorders in humans
such as Zellweger syndrome spectrum.