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1. Mononuclear phagocytes - origins, fates and functions
- Prof. Steffen Jung
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2. Transcription factors in macrophage differentiation
- Dr. Michael Sieweke
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3. Fetal macrophages
- Prof. Paul Martin
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4. The alveolar macrophage
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5. Airway macrophages in health and disease
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6. Intestinal macrophages - heterogeneity, origins and functions
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7. Osteoclasts: what do they do and how do they do it?
- Prof. Steven L. Teitelbaum
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8. The biology of Fc receptors and complement receptors
- Prof. Steven Greenberg
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10. Tumour-associated macrophages
- Prof. Michele De Palma
- Dr. Mario Squadrito
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11. The recognition of pathogens by C-type lectins
- Prof. Gordon D. Brown
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12. Macrophage phagocytosis
- Prof. Joel Swanson
-
13. Macrophage CD36 and atherosclerosis
- Dr. Maria Febbraio
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14. Toll-Like receptor signaling and the innate immune response
- Dr. Kate Fitzgerald
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15. Innate immune receptors as mediators of systemic inflammation and pathogenesis of malaria
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17. Triggering receptors expressed on myeloid cells (TREM)
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18. Influence of eicosanoid lipid mediators on macrophage innate immune functions
- Prof. Marc Peters-Golden
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19. Macrophage paired receptor interactions
- Prof. Neil Barclay
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20. Macrophage heterogeneity in atherosclerosis regression
- Prof. Edward Fisher
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21. Gaucher disease: from lysosomal storage to immunopathology
- Prof. Johannes M.F.G. Aerts
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22. Macrophage in asthma
- Prof. Douglas Robinson
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23. The macrophage mannose receptor
- Dr. Luisa Martinez-Pomares
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24. Myeloid-derived suppressor cells in cancer
- Prof. Dmitry Gabrilovich
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25. EGF-TM7 receptors
- Dr. Jörg Hamann
- Dr. Hsi-Hsien Lin
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26. Macrophages in helminth infection
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- Archived Lectures *These may not cover the latest advances in the field
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27. Immunosuppressive mechanisms in myeloid cells
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Printable Handouts
Navigable Slide Index
- Introduction
- Phagocytosis
- Time-lapse video of a macrophage ingesting cells
- Targets of macrophage phagocytosis
- The importance of macrophage phagocytosis
- Fc receptor-mediated phagocytosis
- Actin cytoskeleton during phagocytosis
- Receptors guide or trigger phagosome formation
- Short & medium range signaling from Fc receptors
- Essential phosphoinositides of phagocytosis
- PI(3,4,5)P3 levels on phagocytic cup membranes
- Fc-gamma receptors
- Actin cytoskeleton moves in phagosome formation
- Movements of the actin cytoskeleton
- Rac signaling and phagocytosis (1)
- Rac signaling and phagocytosis (2)
- Rac1 activation during phagocytosis
- Sources of membrane for phagosome formation
- Patterns of activities in forming phagosomes
- Coordination of phagosome formation
- Target geometry affects phagocytosis
- Coordination of signaling for phagocytosis
- Receptors associated with phagocytosis
- CR3 signaling for phagocytosis
- Phagocytosis of apoptotic cells
- Many routes of entry into cells
- Ruffling & macropinocytosis in response to M-CSF
- Bacteria engulfed in macropinosome-like vacuoles
- Phagosomes change before fusing with lysosomes
- Phagosomes acidify before & after fusion
- Phagosomes mature by sequential interactions
- Microbicidal chemistries in phagosomes
- Phagocyte oxidase activity during phagocytosis
- Manipulation by microbes
- Summary
- Acknowledgments
- References
Topics Covered
- The importance and targets of macrophage phagocytosis
- Phagosome formation & maturation
- Roles of receptors, actin cytoskeleton and phosphoinositides during phagocytosis
- Target geometry effects on phagocytosis
- Signaling for phagocytosis
- Phagocytosis of apoptotic cells
- Ruffling & macropinocytosis
- Microbicidal chemistries in phagosomes
- Manipulation by microbes
Links
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Talk Citation
Swanson, J. (2013, January 30). Macrophage phagocytosis [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 14, 2024, from https://doi.org/10.69645/CGQK5051.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Joel Swanson has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
A selection of talks on Biochemistry
Transcript
Please wait while the transcript is being prepared...
0:00
This talk describing
macrophage phagocytosis
is presented by Joel Swanson
from the Department of
Microbiology and Immunology
at the University of
Michigan Medical School.
0:12
Phagocytosis is the
process by which
cells ingest other
cells or particles,
was a defining feature
of macrophages when they
were first described
in the 19th century.
Macrophages are capable of
ingesting large quantities
of extracellular debris,
particles, or microbes.
Their ability to do
so is essential to
their roles in innate
and adaptive immunity.
The image shown here is a
scanning electron micrograph
of a mouse bone
marrow-derived macrophage
caught in the act of ingesting sheep
red blood cells by phagocytosis.
The macrophage, which has
been pseudocolored blue,
is spread onto a glass surface.
The sheep red blood
cells had been
coated with
immunoglobulin G, or IgG,
to opsonize or prepare them for
the process of phagocytosis.
In this image, the red cells,
pseudocolored red,
are visible in various
stages of engulfment
by extensions of macrophage
plasma membrane.
1:10
This image shows
the same macrophage
viewed by phase-contrast
light microscopy.
Surface protrusions called
ruffles appear as dark,
wavy lines at the
margins of the cell.
The red line indicates
the profile of
a macrophage spread flat
onto the glass cover slip.
This is the first frame of a
time lapse video recording
which shows actual movements
accelerated 100-fold.
The video shows the
active movements
of the surface ruffles
and erythrocytes falling out of
the buffer and onto
the macrophage.
After binding to the cell,
the red blood cells change
from bright to dark as
phagocytic cups from
the macrophage surface
extend out over the particle.
This is particularly evident in
the lower part of the image,
where several erythrocytes are
internalized in rapid succession.
Once internalized, the red cells
remain dark by phase-contrast optics
and move towards the
center of the cell.
Although many kinds of cells
are capable of phagocytosis,