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Printable Handouts
Navigable Slide Index
- Introduction
- What C. elegans are
- Neuronal networks - from stimulation to behavior
- Monitoring neuronal activity - calcium imaging
- In vivo Ca imaging of chemosensory neurons
- Automating In vivo Ca imaging
- An automated calcium imaging platform
- In vivo Ca imaging using a microfluidic chip
- Processing single worms in the microfluidic chip
- Fabrication of the microfluidic chip
- The automated, in vivo calcium imaging platform
- Automated single worm processing on-chip
- Automated calcium imaging on-chip
- A typical Ca response (chemosensory neuron)
- Automated calcium imaging - possibilities
- Effects of age on the function of sensory neurons
- In vivo calcium imaging - parameters
- Age effect on ASH neuronal response
- Peak and slope of the ON response
- Initial intracellular Ca level may be age-dependent
- Calcium pathway in ASH neuron
- Using in vivo imaging for drug screening
- Approach - drug-treated vs untreated worms
- Mianserin - yes, Resveratrol - no
- Summary
- Behind the scene
Topics Covered
- What C. elegans are
- Neuronal networks: from stimulation to behavior
- Monitoring neuronal activity
- Automating In vivo Ca imaging
- An automated calcium imaging platform
- A microfluidic chip
- Processing single worms
- Fabrication
- Automated single worm processing on-chip
- Automated calcium imaging on-chip
- A typical Ca response (chemosensory neuron)
- Effects of age on the function of sensory neurons
- Parameters
- Initial intracellular Ca level may be age-dependent
- Calcium pathway in ASH neuron
- Using in vivo imaging for drug screening
- Drug-treated vs. untreated worms
Talk Citation
Chronis, N. (2012, March 18). Automated Ca2+ imaging of chemosensory neurones in C.elegans [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved December 7, 2024, from https://doi.org/10.69645/AVMV1687.Export Citation (RIS)
Publication History
Financial Disclosures
- Dr. Nikos Chronis has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.