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Printable Handouts
Navigable Slide Index
- Introduction
- Lecture topics
- Role of calcium in plant-environment interaction
- Early cellular evolution
- Calcium concentrations in a plant cell
- Calcium signals in the cell nucleus
- Calcium-regulated gene expression
- Calmodulin and calmodulin-like proteins
- Plant calmodulins and calmodulin-like proteins
- Plant proteins associated with calmodulin
- The CAMTA family
- CAMTA binding sites
- CM2: a binding site required for freezing tolerance
- How calcium regulates transcription
- CaM7 functions as a calcium-regulated TF
- Calcium/CaM-regulated TFs
- CAMTA3: a negative defense responses regulator
- Transcriptome analysis of camta3 mutants
- camta3 T-DNA insertion mutants
- camta3 mutants are resistant to pathogens
- Transcription networks in plant defenses
- Calcium-regulated TFs in abiotic stress response
- Calcium-regulated kinases and phosphatases
- Calcium-dependent thermotolerance
- Cis-elements mediating Ca-regulated transcription
- Bioinformatic analysis of promoters
- Assessment of bioinformatic predictions
- Cis-elements, TFs and target genes (1)
- Cis-elements, TFs and target genes (2)
- Calcium-regulated TFs and plant growth
- Reverse-genetics approaches
- CAMTA1-SRDX
- Hyper-responsiveness to auxin
- CAMTA1 gene expression in abiotic stresses
- Complex interactions among CAMTA genes
- Developmental plasticity model
- Acknowledgements
- References (1)
- References (2)
- References (3)
Topics Covered
- Calcium as a ubiquitous second messenger
- Evolution and status in plant cells
- Calmodulin as a transducer of Calcium signals
- Mechanisms of transcription regulation by Calcium
- Calmodulin-binding transcription activators
- Calcium regulation of transcription in defense responses
- CAMTA and regulation of growth and stress responses
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Talk Citation
Fromm, H. (2020, August 12). Calcium regulation of transcription in plants [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 14, 2024, from https://doi.org/10.69645/HWSL5969.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Hillel Fromm has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
A selection of talks on Biochemistry
Transcript
Please wait while the transcript is being prepared...
0:00
I'm Professor Hillel Fromm, Head of
the department of molecular biology and
ecology of plants at Tel Aviv University.
Today I will talk about calcium
regulation of transcription in plants.
0:12
First, I will give
an overview of the origin and
status of calcium in plant cells.
And I will specifically address
the relationship between the calcium in
the cytosol and in the nucleus.
I will then describe mechanisms by which
calcium regulates gene expression.
And I will further on give examples of
how calcium regulated transcription
is involved in plant defenses,
in biotic and abiotic stresses.
And how calcium regulation of
transcription is involved in hormonal
function and plant growth.
0:40
One of the intriguing properties of
calcium in plant cells is that calcium is
involved with any interaction
of plants with the environment.
This includes the biotic and
abiotic environments.
It includes responses to physical
stimuli like light, heat, cold,
mechanical stress and to chemical stimuli
like salinity, drought and hypoxia.
0:59
The important role of calcium in cellular
life and in plants cells specifically,
that we probably traced back
to early evolution of cell.
Due to the properties of calcium,
it's fast binding to proteins and
other molecules.
It's strong binding, high availability,
and limited solubility.
Early cellular life required ejection
of calcium from the cells to create
a situation where the concentrations
of calcium in the cell is very low.
And thus creating a gradient of four
orders of magnitude of calcium from
outside to inside.
This was probably the initial step to the
evolution of calcium as a signaling system
in plants.
1:38
When we look at the typical plant cells,
we see that the concentration of calcium
in the cytosol it is between
100 to 200 nanomolar.
The outside concentration of calcium in
Apoplast is between one to ten millimolar.
Calcium has the machinery to pump
out calcium from within the cell or
release calcium on the outside of from
an intracellular storage compartments.
And this compartment include chloroplasts,
mitochondria, the And
most importantly in plants the vacuole
which contains concentrations of calcium
to the ten millimolar range.
Upon perception of signals
from the environment,
calcium will be released into
the cytosol either from the outside or
from intracellular compartments, and
will create a calcium signature.
So for each stimulus from the outside will
be a different pattern of calcium that is
called a calcium signature.
The question now is how genes
are regulated by calcium.
Since the genes are in the nucleus,
we have to ask the questions of what
happens with calcium in the nucleus.
The nucleus is not a sealed envelope but
actually contains pores that can even
let proteins go into the nucleus.
So for many years it was assumed that
calcium simply moves freely through these
pores into the nucleus.
However, now it's believed that calcium
cannot move freely through these pores.
It may go through these pores
under certain circumstances.
However the most interesting thing is
that the nucleus has calcium storage and
release machinery in the nuclear envelope.