• Introduction
• 38 min
  1. DNA methylation during development

  • Prof. Howard Cedar
• The Nuts and Bolts of DNA Methylation
• 54 min
  2. DNA methylation patterns in mammals

  • Prof. John Greally
• 31 min
  3. Proteins that bind methylated DNA

  • Dr. Pierre-Antoine Defossez
• DNA Methylation and Normal Physiology
• 25 min
  4. Genomic imprinting

  • Prof. Marisa Bartolomei
• 32 min
  5. DNA methylation and stem cells

  • Dr. Alexander Meissner
• DNA Methylation and the Pathophysiology of Disease
• 54 min
  6. DNA methylation changes in aging cells and tissues

  • Prof. Jean-Pierre Issa
• 61 min
  7. DNA methylation in cancer

  • Prof. Paula Vertino
• 29 min
  8. Epigenomics of complex disease

  • Prof. Art Petronis
• 41 min
  9. DNA methylation, inflammation and cancer

  • Prof. Gerd Pfeifer
• Clinical Applications of DNA Methylation Research
• 41 min
  10. DNA methylation as a tumor marker: implications for diagnosis, early detection and risk assessment

  • Prof. Steve Belinsky
• 46 min
  11. Reversing DNA methylation pharmacologically

  • Dr. Allen Yang

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Genomic imprinting overview
3. Nuclear transplantation in the mouse
4. Uniparental development in humans
5. Uniparental disomy
6. Mouse imprinted genes, regions and phenotypes
7. Human and mouse imprinted regions
8. The mark of imprinted genes
9. Properties of the mark
10. Candidates for the mark
11. Is DNA methylation the imprinting mark?
12. H19 and Igf2 imprinted locus
13. The mouse H19 gene is differentially methylated
14. DNA methylation at the H19 locus
15. H19 and Igf2 share enhancers
16. Deletion of Dnmt1 activates the paternal H19
17. H19 is biallelically expressed in Dnmt1 -/- embryos
18. Insulator mechanism
19. Insulators: barriers and enhancer blockers
20. Insulator model for imprinted genes - H19 Igf2 locus
21. Long non-coding RNA mechanism of imprinting reg.
22. Imprinted genes summary
23. When and how are genes marked in the germline?
24. DNA methylation erasure in embryonic germ cells
25. Methylation at the Snrpn ICR in growing oocytes
26. The hypothesis
27. How are imprints maintained?
28. Summary

Topics Covered

• Genomic Imprinting: Definition of genomic imprinting
• Why genomic imprinting is essential for development
• Imprinted genes
• Mechanism of genomic imprinting
• H19/Igf2 imprinted locus
• Marking of imprinted genes in the germline
• Maintenance of genomic imprints

Links

Series:

• DNA Methylation

Categories:

• Cell Biology
• Genetics & Epigenetics

Talk Citation

Publication History

• Published on March 3, 2011

Financial Disclosures

• Professor Marisa Bartolomei has no commercial/financial relationships to disclose