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- Basic Concepts
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1. Molecular structure of the human genome
- Prof. Jonathan Wolfe
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2. Introduction to mutation and disease
- Prof. Jonathan Wolfe
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3. Introduction to genetic diseases
- Prof. Jonathan Wolfe
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4. Historical introduction to human diversity I
- Prof. Dallas Swallow
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5. Historical introduction to human diversity II
- Prof. Dallas Swallow
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6. Introduction to cytogenetics 1
- Dr. Sioban SenGupta
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7. Introduction to cytogenetics 2
- Dr. Sioban SenGupta
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8. Introduction to cytogenetics 3
- Dr. Sioban SenGupta
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9. The Y-chromosome
- Prof. Mark Jobling
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10. Mitochondrial DNA, molecular genetics and human mitochondrial diseases
- Prof. Immo Scheffler
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11. Mechanisms of DNA repair by recombination
- Prof. James Haber
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12. Techniques utilized in molecular genetics
- Dr. Cecil Lewis
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13. Genome mapping
- Dr. Simon Gregory
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14. Copy number variation
- Dr. Ömer Gökçümen
- Genetics and Disease
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15. Genetic association studies
- Prof. David Balding
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16. Quantitative genetics and multifactorial inheritance
- Dr. Lara Bauman
- Human Evolutionary Genetics
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17. Population genetics
- Dr. Murray P. Cox
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18. Inference on human history through DNA
- Prof. Guido Barbujani
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19. Human adaptation
- Prof. Rasmus Nielsen
- Specialized Topics
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20. Accessing and using ENCODE data
- Prof. Peggy Farnham
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21. Pharmacogenetics
- Prof. Ann Daly
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22. Genetics in forensics
- Dr. Angel Carracedo
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23. The development and evolution of human gene therapy
- Prof. Theodore Friedmann
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24. Next generation sequencing technologies
- Dr. Krishna Veeramah
Printable Handouts
Navigable Slide Index
- Introduction
- Genome instability in tumor cells
- The "Philadelphia chromosome"
- Loss of heterozygosity (LOH)
- Defects in repair of chromosome breaks
- Double strand break (DSB) repair is essential
- Talk outline
- DSB can arise by replication over a ssDNA nick
- Stalled replication forks may "break"
- DSBs at "fragile sites"
- Oncogene-induced DNA damage model
- Programmed DSBs
- Burkitt's lymphoma - common translocation
- Homologous recombination mechanisms (1)
- Replication fork regression
- EM of regressed replication fork
- Holliday junctions (HJ) - EM
- HJ is a symmetric structure
- HJ can branch migrate
- RuvA and RubB drive branch migration
- Branch migration in E.coli
- HJ are "resolved" by enzymes (1)
- HJ are "resolved" by enzymes (2)
- Cleavage of a HJ at a stalled replication fork
- Replication re-start by homologous recombination
- Basic strand exchange (1)
- Strand exchange - in vitro assays
- Basic strand exchange (2)
- Basic strand exchange (3)
- X-ray crystallography of strand exchange
- Resolution of remaining HJs
- Sisters chromatids exchange (SCE)
- DNA damage induces SCE events
- Break induced replication (BIR)
- BIR and telomerase
- Homologous recombination mechanisms (2)
- Single strand annealing
- Single strand annealing can produce deletions
- Homologous recombination mechanisms (3)
- DNA repair by gene conversion
- Bloom's syndrome
- Synthesis-dependent strand annealing (SDSA)
- Budding yeast as a model of SDSA
- Observing DNA repair in real time
- DSB repair process (1)
- DSB repair process (2)
- Visualizing Rad51 loading
- Mutagenesis associated with gene conversion
- Overview of DNA repair mechanisms
- Nonhomologous end-joining pathways (NHEJ)
- Ku- and Xrcc4-independent alternatives
- NHEJ plays a major role in translocations
- Changes in copy number in cancer cells
- Gene amplification
- Copy number variation involves recombination
- Summary
- Acknowledgements
Topics Covered
- Genome instability in tumor cells
- Mutations
- Loss of heterozygozity
- Repair of chromosome breaks
- Double-strand break repair is essential for life
- Replication stalling and fragile sites
- Programmed DSBs
- Repair of DSBs arising during DNA replication by recombination
- The homologous recombination machinery
- E. coli
- Holliday junctions
- Break-induce replication
- Single strand annealing
- Types of homologous recombination
- Observing DNA repair in real time
- Molecular studies of recombination
- Nonhomologous end-joining (NHEJ) pathways
- Copy number variation
- Gene amplification
Talk Citation
Haber, J. (2009, December 28). Mechanisms of DNA repair by recombination [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved November 14, 2024, from https://doi.org/10.69645/QDUR1534.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. James Haber has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.