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- Co-ordination of G1 Progression
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1. START control in yeast
- Prof. Curt Wittenberg
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2. The pRB/E2F pathway
- Prof. Jacqueline Lees
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3. Cell cycle control by the ubiquitin system in mammals
- Prof. Michele Pagano
- Chromosome Duplication
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4. Replication licensing
- Prof. Julian Blow
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5. Initiation of DNA replication
- Prof. Bruce Stillman
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6. Regulation of replication fork progression and stability
- Dr. Luis Aragón
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7. Nucleosome assembly during DNA replication
- Dr. Alain Verreault
- Preparing for Mitosis
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8. Sister chromatid cohesion: simple concept, complex reality
- Prof. Douglas Koshland
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9. Mitotic chromosome condensation
- Prof. Andrew Belmont
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10. Centrosome duplication and separation in animal cells
- Prof. Andrew Fry
- Spindle Assembly and Chromosome Segregation
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11. Bipolar spindle assembly
- Dr. Eric Karsenti
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12. Chromosome bi-orientation in yeast
- Prof. Mike Stark
- Prof. Tomo Tanaka
- Mitotic Exit and Cytokinesis
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14. Cleavage furrow formation and ingression during animal cytokinesis
- Dr. Pier Paolo D'Avino
- Checkpoints Governing Cell Cycle Progression
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15. The DNA damage response
- Dr. Vincenzo Costanzo
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16. The spindle checkpoint
- Dr. Kevin Hardwick
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17. Spindle movement and checkpoint control during mitosis in yeast
- Prof. John Cooper
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18. The G2/M transition
- Prof. Dr. René Medema
- The Cell Cycle in Development and Cancer
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19. Mouse models to investigate cell cycle and cancer
- Dr. Philipp Kaldis
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20. Cell cycle: a complex network of signals regulating cell proliferation
- Prof. Antonio Giordano
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21. Drug discovery and target validation in the p53 pathway
- Prof. Sir David Lane
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22. Role and regulation of Cdk inhibitors in development and cancer
- Prof. Martine Roussel
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24. The Myc transcription factor network
- Prof. Robert N. Eisenman
- Meiosis: A Specialized Cell Cycle
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25. Recombination and the formation of chiasmata in meiosis
- Prof. Matthew Whitby
- Archived Lectures *These may not cover the latest advances in the field
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26. Geometric regulation of kinetochore orientation
- Prof. Yoshinori Watanabe
Printable Handouts
Navigable Slide Index
- Introduction
- Outline
- Perspective: million fold multiplier
- Evolutionary and developmental comparisons
- Phenomenology: stages of mitosis
- Stages of mitosis (1)
- Stages of mitosis (2)
- Primary functions of mitosis
- Chromosome length and chromosome segregation
- Lower levels of chromatin organization
- Two common observations
- Early whole mount electron microscopy
- Helical folding at highest level of compaction
- What we thought we knew
- Key advance
- Micrographs of mitotic chromosomes
- Metaphase chromatid cross section
- The radial loop model
- Interpretation
- SARs and MARs
- DNA sequences at the base of chromatin loops
- Nonhistone proteins make up scaffold
- Sc1 = topoisomerase 2
- Condensin complex containing Sc2 (1)
- Condensin complex containing Sc2 (2)
- Condensin complex containing Sc2 (3)
- Condensin complex containing Sc2 (4)
- SMC family of proteins
- Vindication of radial loop/scaffold model
- Textbook model: condensation by condensins
- Challenges to textbook model (1)
- Challenges to textbook model (2)
- 200-300 nm subunit in metaphase chromosomes
- Alternative model - Radial loop, Helical coil model
- SMC2 gold staining doesn't support the coiling
- Challenges to the textbook model (3)
- Topo 2 dependant chromosome condensation
- Chromosome condensation with topo 2 knockdown
- Chromosome condensation with SMC2 knockdown
- What we think we know
- Phenotype of topo 2 and condensin knockdown
- Condensins is required for chromosome stability
- Two condensin complexes are present
- Condensin 1 versus 2
- What we'd like to know
- Chromosome scaffold versus "glue" model
- Possibilities for condensin knockdown results
- Condensin activity and chromosome structure
- Alternative model for chromosome condensation
- Model
- Structural studies and chromosome biochemistry
- Chromosome structure: remaining questions (1)
- Chromosome structure: remaining questions (2)
- Recommended reviews
Topics Covered
- Evolutionary and developmental comparisons
- Stages of mitosis
- Primary functions of mitosis
- Lower levels of chromatin organization
- Helical folding
- Experimental foundation of textbook model
- Are there specific DNA sequences at the base of chromatin loops?
- Are there specific nonhistone proteins making up the chromosome scaffold?
- Three independent experimental paths led to identification of condensin complex containing Sc2
- SMC family of proteins
- Condensation by condensins
- Challenges to textbook model
- progressive condensation during prophase
- Alternative model: Radial loop, helical coil model
- What we think we know
- What we'd like to know
- Alternative mechanism for chromosome condensation
- How do we integrate structural studies of chromosome structure with chromosome biochemistry
- We need a better understanding of chromosome structure
Talk Citation
Belmont, A. (2009, September 3). Mitotic chromosome condensation [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 20, 2024, from https://hstalks.com/bs/1426/.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Andrew Belmont has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.