Methods to Study GPCRs and Applications of GPCR Engineering

Launched January 2020 3 lectures
Dr. M. Madan Babu
MRC Laboratory of Molecular Biology, UK

Since the development of the radio ligand binding techniques to study GPCRs new methods have had a significant impact in helping us understand how this class of proteins function at different scales: from the atomic scale to that of the whole organism.... read more

The key objective of this series is to describe and discuss the various approaches that
scientists use to investigate GPCRs in order to understand their mechanism of action,
emergent properties of GPCR signalling and their role physiology and disease. It will
provide knowledge of fundamental principles that underlies different methodologies and highlight how they can shed new light on GPCR signalling. This series will be of significant interest to undergraduates, graduates, academic and industry researchers, medics, physicians and methods developers who are new to the field of GPCRs. It will also provide up-to-date knowledge for researchers already working in this area.

The first set of talks will describe computational approaches to investigate GPCRs with an emphasis on storing, integrating and mining information, as well as computational modelling of receptors and mathematical modelling of signalling pathways. The next set of talks will discuss molecular dynamics and experimental approaches to investigate GPCR conformational dynamics as well as the oligomeric complexes that they form. The third will cover various biophysical methods such as mass spectrometry, single molecule analysis, proximity labelling in cells, Hydrogen-Deuterium exchange, solid-state NMR spectroscopy among other approaches. The fourth focuses on methods and approaches for structure determination; from protein engineering and purification to elucidation of the atomic structure of receptors using different approaches such as crystallography, NMR and Cryoelectron microscopy. The fifth set will focus on imaging approaches to study GPCR signalling; from in vitro to in cell and whole organism imaging. The final set of talks will discuss methods that allow engineering GPCR signalling to investigate and control cellular processes and understand human diseases such as cancer.