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1. Introduction
2. Stem cells undergo asymmetric self-renewing
3. Why stem cells are important
4. Stem cells in Drosophila
5. Cell types in the Drosophila ovary
6. Markers expressed in specific cell types
7. Lineage tracing (1)
8. Lineage tracing (2)
9. Lineage tracing (3)
10. Stem cell niche
11. Expanded niche expand stem cell population
12. The GAL4/UAS system of gene expression
13. Overexpression of dpp expands GSC population
14. Dpp pathway in Drosophila
15. GSC loss in dpp pathway mutants
16. Clonal analysis in Drosophila
17. Downstream components of dpp pathway
18. Model for Dpp regulation of GSCs
19. Lost GSCs are replaced
20. Cell types in the Drosophila testis
21. upd is expressed in Hub
22. Jak/Stat pathway in Drosophila
23. Disruption of Jak/Stat results in GSC loss
24. Over-expression of upd
25. Model of testis niche
26. Niche maintains GSC
27. Can cysts revert back to stem cells? (1)
28. Can cysts revert back to stem cells? (2)
29. Stat mutant cells exhibit precocious development
30. Differentiated cysts breakdown and repopulation
31. Niches maintain GSCs and drive dedifferentiation
32. Do stem cells signal back to niche?
33. Notch Delta signaling pathway
34. Activation of Notch pathways
35. Loss of Notch signaling leads to loss of GSCs
36. Drosophila digestive tract
37. Clonal analysis
38. Midgut has multipotent stem cells
39. Notch signaling drives differentiation in midgut
40. Silencing of differentiation factors in stem cells
41. Expression of nuclear proteins in stem cells
42. scny shares homology with yeast and USPs
43. scny mutant cells display elevated H3mK4 levels
44. scny mutants exhibit chromatin changes
45. scny mutants exhibit stem cell loss in ovary (1)
46. scny mutants exhibit stem cell loss in ovary (2)
47. scny functions in follicle stem cells
48. Cell lineage in the Drosophila adult midgut
49. DAPT blocks Notch activity in midgut
50. DAPT suppresses stem cell loss in scny midguts
51. Model for SCNY function in stem cells
52. Take home messages
53. Acknowledgments
54. References for specific images

Topics Covered

• Lineage tracing
• Stem cell niches
• Germline stem cell niche in ovary
• Germline stem cell niche in testis
• Dedifferentiation
• Stem cell signaling back to the niche
• Intestinal stem cells
• Stem cell chromatin

Links

Series:

• The Legacy of Drosophila Genetics

Categories:

• Cell Biology

Talk Citation

Publication History

• Published on September 29, 2008

Financial Disclosures

• Prof. Michael Buszczak has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.