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Navigable Slide Index

1. Introduction
2. Mitochondria and reactive oxygen species
3. Oxygen chemistry
4. Reactive oxygen species chemistry
5. ROS mediated cellular damage
6. Reactive oxygen species in disease
7. Antioxidant defenses
8. Overview of mitochondrial ROS generation (1)
9. Mitochondria structure and topology
10. Mitochondria: a cellular source of oxygen radicals
11. Respiratory complexes
12. Mitochondria bioenergetics
13. ROS generation by mitochondria - overview
14. ROS generation by mitochondria - chemistry
15. ROS generation by mitochondria
16. Sites and mechanism of ROS generation
17. Complex I
18. Complex II
19. Complex III
20. Ubisemiquinone
21. Complex IV
22. Antioxidant defenses - superoxide dismutase
23. Antioxidant defenses - mitochondrial GSH
24. Superoxide generated towards the matrix
25. Superoxide generated towards the IM space
26. Superoxide anion diffusion control through VDAC
27. VDAC control the release of superoxide to cytosol
28. Topology of ROS generation by mitochondria
29. Literature of topology of superoxide release
30. Mitochondria - a source of oxygen radicals
31. Overview of mitochondrial ROS generation (2)
32. Methods to measure hydrogen peroxide release
33. Use of DCFH to measure hydrogen peroxide
34. Methods to measure superoxide release
35. Measurement of superoxide using hydroethidine
36. Overview of mitochondrial ROS generation (3)
37. Regulation of mitochondrial ROS generation (1)
38. Respiration rate by the electron transport chain (1)
39. UCP as a mitochondrial ROS generation regulator
40. Respiration rate by the electron transport chain (2)
41. Respiration rate by the electron transport chain (3)
42. Regulation of mitochondrial ROS generation (2)
43. Mitochondrial GSH
44. GSH depletion and complex I H2O2 production
45. Effect of GSH depletion on H2O2 production
46. Regulation of mitochondrial ROS generation (3)
47. Ceramides
48. Ceramide and mitochondrial ROS generation
49. Regulation of mitochondrial ROS generation (4)
50. NO - a regulator of mitochondrial function
51. Inhibition of mitochondrial respiration by NO
52. Inhibition of cytochrome oxidase by NO
53. Formation of peroxynitrite
54. Biphasic effect of NO on H2O2 production
55. Reactive oxygen and nitrogen species generation
56. Regulation of mitochondrial ROS generation (5)
57. Post translational protein modifications
58. Mitochondrial post translational modifications
59. S-glutathionylation of aconitase decreases activity
60. Post translational modification of complex I
61. Modifying the cell's redox status
62. Post translational modification by ROS and RNS
63. Signaling molecules and MAPK regulation
64. Summary
65. Acknowledgements

Topics Covered

• Oxygen chemistry
• Reactive oxygen species chemistry and in disease
• Antioxidants defenses
• Mitochondria structure and topology
• Mitochondria bioenergetics
• Sites and mechanisms
• Superoxide dismutase
• Mitochondrial GSH
• Superoxide generation
• Voltage-dependent anion channels
• Methods to measure reactive oxygen species
• Regulation of mitochondrial reactive oxygen species generation
• Rate of respiration by the electron transport chain
• Ceramide
• Nitric oxide
• Post-translational modification

Links

Series:

• Free Radicals and the Oxygen Paradox

Categories:

• Biochemistry

Talk Citation

Cadenas, E. and Han, D. (2007, November 1). Generation of reactive oxygen species by mitochondria [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 23, 2024, from https://hstalks.com/bs/378/.
Export Citation (RIS)

Publication History

• Published on November 1, 2007
• Archived on May 31, 2018

Financial Disclosures

• Dr. Enrique Cadenas has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
• Dr. Derick Han has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.