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1. Introduction
2. Sanger sequencing
3. Sanger limitations
4. The Archon Genomics Xprize
5. Sequencing by synthesis
6. 454 sequencing by Roche
7. Sample preparation: DNA fragmentation
8. Addition of adaptors
9. Adaptors A and B
10. Isolation of fragments with only one A and one B
11. Binding of the DNA fragments to capture beads
12. Formation of 'micro-reactors'
13. Clonal amplification step
14. Addition of the sequencing primer
15. Loading the beads onto a PicoTiter plate
16. Sequencing reaction
17. Illustration of the sequencing reaction
18. Flowgram
19. Assembly of the entire target sequence
20. Solexa sequencing by Illumina
21. Sample preparation for Solexa sequencing
22. Fixation of the strands onto a flow cell
23. Formation of a DNA fragment bridge
24. Creation of a double-stranded bridge by PCR
25. Formation of single-stranded DNA array
26. Clonal amplification of DNA clusters
27. Formation of the sequencing templates
28. Reversible terminators
29. The sequencing reaction of Solexa technology (1)
30. Laser excitation
31. The sequencing reaction of Solexa technology (2)
32. Analysing flow cell images
33. End result of Solexa sequencing
34. SOLid sequencing by ABI
35. Sample preparation for SOLid sequencing
36. Deposition of the DNA beads onto glass slide
37. Pre-sequencing step of SOLid technology
38. The probes (1)
39. The probes (2)
40. The sequencing reaction of SOLid technology
41. Detecting a single colour does not indicate a base
42. Colour code gives multiple possibilities
43. Decoding the code using base-pair "0" (1)
44. Decoding the code using base-pair "0" (2)
45. End result of SOLid sequencing
46. Stats for the 3 main competitors
47. Some other methods
48. True single molecule sequencing
49. Sequencing by hybridization
50. Nanopore sequencing
51. Real time polymerase sequencing
52. Further reading

Topics Covered

• Sanger sequencing
• The Archon Genomics Xprize
• Sequencing by synthesis 454 sequencing by Roche
• Solexa sequencing by Illumina
• SOLid sequencing by ABI
• Stats for the 3 main competitors
• Some other methods
• True single molecule sequencing
• Sequencing by hybridization
• Nanopore sequencing
• Real time polymerase sequencing

Links

Series:

• Introduction to Human Genetics

Categories:

• Genetics & Epigenetics
• Methods

Talk Citation

Publication History

• Published on December 30, 2009

Financial Disclosures

• Dr. Krishna Veeramah has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.