• Introduction and General Properties of Plasmids
• 31 min
  1. Introduction to plasmid biology

  • Prof. Emeritus Don Clewell
• 39 min
  2. General properties and applications of plasmids

  • Prof. Emeritus Don Clewell
• Replication
• 47 min
  3. Plasmids: copy number control by antisense RNAs

  • Prof. Gerhart Wagner
• 30 min
  4. Plasmid rolling-circle (RC) replication

  • Prof. Saleem Khan
• 45 min
  5. Replication of linear plasmids in bacteria

  • Prof. George Chaconas
• Maintenance and Partitioning
• 32 min
  6. Plasmid segregation and stability in bacteria

  • Prof. Barbara Funnell
• 33 min
  7. Plasmid stabilization by cell killing

  • Prof. Keith Weaver
• 30 min
  8. Dimer catastrophes and plasmid resolution

  • Dr. David Summers
• Conjugation and Mobilization
• 41 min
  9. The diversity of bacterial conjugation and mobilization systems

  • Prof. Fernando de la Cruz
• 36 min
  10. The pCF10-encoded pheromone response: cell-cell signaling, complexity and evolution

  • Prof. Gary Dunny
• 27 min
  11. DNA processing in conjugative transfer

  • Dr. Joel Schildbach
• 43 min
  12. Agrobacterium tumefaciens

  • Dr. Stephen Winans
• Genetic Traits Frequently Associated with Plasmids
• 37 min
  13. Insertion sequences and DNA transposition

  • Prof. William Reznikoff
• 48 min
  14. Plasmids, integrons and the spread of antibiotic resistance

  • Prof. Hatch Stokes
• 30 min
  15. Virulence plasmids

  • Prof. Julian Rood
• Eukaryotic Plasmids
• 30 min
  16. The yeast plasmid: a highly persistent selfish DNA element

  • Prof. Makkuni Jayaram
• Population Biology and Evolution
• 35 min
  17. Evolution of plasmids and their role in bacterial diversity and adaptability

  • Prof. Christopher Thomas
• Archived Lectures *These may not cover the latest advances in the field
• 85 min
  18. Bacterial extrachromosomal elements: role in chemical detoxification and potential cancer therapy

  • Prof. Ananda Chakrabarty
• 34 min
  19. Plasmid segregation and stability in bacteria

  • Prof. Barbara Funnell
• 39 min
  20. Replication of linear plasmids in bacteria

  • Prof. George Chaconas

Printable Handouts

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Navigable Slide Index

1. Introduction
2. Agrobacterium tumefaciens
3. Agrobacterium infection and the Ti plasmid
4. Infection and colonization
5. Transfer, integration and expression of T-DNA
6. Function of some transfered genes
7. Evolution of T-DNA transfer from conjugation
8. Using Agrobacterium to create "transgenic" plants
9. Expression of toxin from Bacillus in plants
10. Detection of plant-released signals by VirA-VirG
11. Representative Vir-inducing phenolic compounds
12. VirA structure and function
13. VirH2 activity
14. Quorum sensing
15. LuxR-LuxI system of Vibrio fisheri
16. TraR-TraI system of Agrobacterium
17. Activation of repABC system
18. Regulation of Ti plasmid copy number
19. Synthesis of AHL by TraI
20. Agrobacterium AttM protein
21. AttM is part of the attKLM operon
22. TraR-autoinducer complex
23. TraR as a class I or class II activator
24. Purification and solubility of TraR
25. OOHL stabilzes TraR
26. Formation of TraR-OOHL-DNA complex
27. Crystal structure of TraR-AHL-DNA complex
28. AHL is engulfed by TraR to it's hydrophobic core
29. TraR activity is antagonized by TraM
30. Binding of TraR to DNA with dyad symmetry
31. Essential bases in TraR binding site
32. Affinity of TraR for wild type tra box and variations
33. TraR residues required for transcription activation
34. TraR function in concert with other factors
35. Activation of traA and traC promoters by TraR
36. The tra box sequence
37. Repression of traA and traC promoters
38. Summary

Topics Covered

• Agrobacterium and tumor inducing plasmid
• T-DNA transfer
• Using Agrobacterium to create "transgenic" plants
• VirA and VirG
• Two component system
• Quorum sensing
• LuxR-LuxI-like regulatory system composed of TraR and TraI
• Acylhomoserine lactones (AHLs)
• The crystal structure of TraR
• TraR and expression of target promoters

Links

Series:

• Biology and Significance of Plasmids

Categories:

• Genetics & Epigenetics
• Methods
• Microbiology

Therapeutic Areas:

• Infectious Diseases

Talk Citation

Publication History

• Published on January 28, 2008

Financial Disclosures

• Dr. Stephen Winans has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.