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- Introduction and General Properties of Plasmids
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1. Introduction to plasmid biology
- Prof. Emeritus Don Clewell
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2. General properties and applications of plasmids
- Prof. Emeritus Don Clewell
- Replication
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3. Plasmids: copy number control by antisense RNAs
- Prof. Gerhart Wagner
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4. Plasmid rolling-circle (RC) replication
- Prof. Saleem Khan
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5. Replication of linear plasmids in bacteria
- Prof. George Chaconas
- Maintenance and Partitioning
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6. Plasmid segregation and stability in bacteria
- Prof. Barbara Funnell
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7. Plasmid stabilization by cell killing
- Prof. Keith Weaver
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8. Dimer catastrophes and plasmid resolution
- Dr. David Summers
- Conjugation and Mobilization
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9. The diversity of bacterial conjugation and mobilization systems
- Prof. Fernando de la Cruz
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11. DNA processing in conjugative transfer
- Dr. Joel Schildbach
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12. Agrobacterium tumefaciens
- Dr. Stephen Winans
- Genetic Traits Frequently Associated with Plasmids
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13. Insertion sequences and DNA transposition
- Prof. William Reznikoff
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14. Plasmids, integrons and the spread of antibiotic resistance
- Prof. Hatch Stokes
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15. Virulence plasmids
- Prof. Julian Rood
- Eukaryotic Plasmids
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16. The yeast plasmid: a highly persistent selfish DNA element
- Prof. Makkuni Jayaram
- Population Biology and Evolution
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17. Evolution of plasmids and their role in bacterial diversity and adaptability
- Prof. Christopher Thomas
- Archived Lectures *These may not cover the latest advances in the field
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19. Plasmid segregation and stability in bacteria
- Prof. Barbara Funnell
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20. Replication of linear plasmids in bacteria
- Prof. George Chaconas
Printable Handouts
Navigable Slide Index
- Introduction
- Outline
- Transfer of DNA between bacterial cells
- Basic plasmid platforms
- Tra vs. Mob: R388 and RSF1010
- The mechanism of conjugation
- The key components in conjugation
- TrwB - a coupling protein
- Related DNA transport processes
- The mating channel
- Liquid vs. surface maters
- The relaxosome
- Shoot and pump conjugation model
- The complex between TrwC and its substrate DNA
- Catalytic center mutagenesis
- TrwC relaxase protein family
- Conserved relaxase motifs in six relaxase families
- 3D relaxase structures
- Diversity and classification of conjugation systems
- The IncW family
- IncW backbone
- Segregational incompatibility
- Methods for plasmid classification
- Well known plasmid Inc groups in Enterobacteria
- Conserved relaxase motifs in six relaxase families
- BLAST searches against prototype relaxases
- MOB-P relaxase family
- The MOB-F family
- MOB-H relaxase family
- MOB-C family
- Classification by relaxases vs. T4CP and T4SS
- Plasmid MOB families
- MOB families distribution and relaxase signatures
- A search on the NCBI plasmid database
- Progression in plasmid sequencing
- Plasmid dstribution in bacterial host phyla
- Plasmid distribution according to size
- Conjugative vs. mobilizable plasmids
- Relaxase families in 1,010 plasmids
- MOBs distribution according to plasmid size
- Relaxases distribution in main bacterial phyla
- Relaxases dstribution in phylum Proteobacteria
- NCBI plasmid database analysis: conclusions
- Acknowledgements
Topics Covered
- Transfer of DNA between bacterial cells
- Basic plasmid platforms
- The mechanism and key components of conjugation
- The mating channel
- The complex between TrwC and its substrate DNA
- The relaxosome
- The diversity of relaxases
- The IncW family
- Methods for plasmid classification
- Plasmid MOB families
- The NCBI plasmid database
- Plasmid sequencing
Links
Series:
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Talk Citation
de la Cruz, F. (2008, January 28). The diversity of bacterial conjugation and mobilization systems [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 31, 2024, from https://doi.org/10.69645/AUCI8961.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Fernando de la Cruz has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.