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1. P450s catalytic cycle
2. Reaction catalyzed by P450
3. Catalytic cycle - reviews
4. The catalytic cycle
5. Catalytic cycle - side reactions
6. Substrate binding
7. Substrate binding to CYP BM3
8. Substrate binding to CYP2C9
9. Oxygen cleavage & substrate hyd.
10. Catalytic cycle - electron transfer
11. Electron sources
12. P450 monooxygenase in the ER
13. NADPH-P450 reductase (CPR)
14. CPR - cofactors
15. CPR - kinetics & thermodynamics
16. Interflavin electron transfer - T-jump
17. Interflavin electron transfer - rates
18. Electron transfer viscosity effect
19. CPR - domain motion
20. Coenzyme effects on domain motion
21. Cytochrome b5
22. b5 and P450s
23. b5 - electron transfer
24. b5 - allosteric effects & summary
25. Acknowledgements

Topics Covered

• Catalytic cycle of cytochrome P450s
• Substrate and oxygen binding
• Key electron and proton transfers
• Dioxygen cleavage
• Formation of the 'activated oxygen' hydroxylating species
• Structure and electron transfer properties of NADPH-cytochrome P450 reductase
• Possible roles of cytochrome b5 in the p450 monooxygenase system

Links

Series:

• Drug Metabolizing Enzymes

Categories:

• Biochemistry
• Metabolism & Nutrition
• Pharmaceutical Sciences

Therapeutic Areas:

• Cardiovascular & Metabolic

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on August 14, 2015

Financial Disclosures

• Prof. Gordon Roberts has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.