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- Principles in Bacterial Pathogenesis
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1. The molecular basis of bacterial pathogenicity: an overview
- Prof. B. Brett Finlay
- Gram Negative Pathogens
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2. Deciphering shigella invasion of epithelial cells
- Prof. Philippe Sansonetti
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5. Bordetella pertussis
- Prof. Alison Weiss
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6. Salmonellae: molecular basis of infection
- Prof. Samuel Miller
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7. The diversity of Escherichia coli infections
- Prof. Michael Donnenberg
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8. Bacterial activation of epithelial signaling
- Prof. Alice Prince
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9. Human pathogenic Yersinia species
- Prof. James Bliska
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11. Dental pathogens
- Prof. Ann Progulske-Fox
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12. Haemophilus
- Prof. Robert Munson
- Gram Positive Pathogens
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13. The bacterial pathogen Listeria monocytogenes: an amazing multifaceted model
- Prof. Pascale Cossart
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15. Molecular pathogenesis and prevention of Staphylococcus aureus infections
- Prof. Olaf Schneewind
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16. Streptococcus pyogenes disease and molecular pathogenesis
- Prof. P. Patrick Cleary
- Host Responses
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17. Microbial recognition and the immune response
- Dr. Dana Philpott
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18. Enteric pathogens-microbiota-host inter-kingdom chemical interactions
- Prof. Vanessa Sperandio
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19. Toll-like receptor signalling during infection and inflammation
- Prof. Luke O'Neill
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20. The human indigenous microbiota
- Prof. David Relman
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22. Role of neutrophils in acute lung injury and repair
- Prof. Gregory Downey
- Preventatives and Therapeutics
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23. Antibiotics and antibiotic resistance
- Prof. Gerry Wright
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24. Vaccines: a health insurance of the 21st century
- Prof. Rino Rappuoli
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25. Biodefense vaccines
- Prof. James Nataro
- Archived Lectures *These may not cover the latest advances in the field
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26. Vaccines in the modern world
- Prof. Gordon Dougan
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27. Bacterial infection of epithelial signaling
- Prof. Alice Prince
Printable Handouts
Navigable Slide Index
- Introduction
- Recognition of bacteria by airway epithelial cells
- A cystic fibrosis patient's airway
- Questions that need to be addressed
- Epithelial cells express toll like receptors
- Signaling through the TLR2
- Characteristics of airway epithelial cells
- Epithelial cells' response to presence of bacteria
- Bacteria stimulate mobilization of receptors
- Questions of interest
- Calcium as a second messenger
- How is the Calcium (Ca2+) released in the cell?
- TLR2 stimulation mediates the release of Ca2+
- Bacterial Ca2+ fluxes induction is TLR2 dependent
- Ca2+ release signals proinflammatory responses
- Bacterial induction of NF-kB is TLR2 dependent
- TLR2 stimulation signals Ca2+ fluxes
- PI3K is required for TLR2 mediated Ca2+ fluxes
- PLC-G is required for TLR2 mediated Ca2+ fluxes
- Summary of the process described so far
- Junctional components of epithelial cells
- Does Ca2+ act as an inter-cellular messenger?
- Connexins and gap junctions
- Connexin's structural organization
- Signaling moves from cell to cell via gap junctions
- Gap junction inhibition attenuates IL-8 secretion
- Do TLR2 Ca2+ fluxes travel through gap junctions?
- Airway cells communicate TLR2 signals via GJ
- Is GJ communication biologically significant?
- What is a possible mechanism for this regulation?
- Cell communication is decreased after stimulation
- Calcium involvement in epithelial signaling
- P. aeruginosa modifies epithelial permeability
- Occludin, E-cadherin altered in response to PAO1
- PAO1 (heat killed) does not alter barrier properties
- Epithelial junctions
- Occludin and E-cadherin
- Epithelia signaling modulates junction properties
- Does calpain target junctional proteins?
- Calpain is a Ca2+ dependent protease
- Calpain directly targets specific junctional proteins
- Calpain activation in airway epithelial cells
- TLR2 dependent calpain activation
- Calpain association with Occludin
- Occludin is cleaved by calpain
- Decrease in hyperphosphorylated Occludin
- Where is Occludin cleaved?
- Calpain association with E-cadherin
- Does calpain change epithelial junction properties?
- Calpain and PMN transepithelial migration
- Calpain activity contributes to inflammation in vivo
- Does calpain help recruit PMN to the airways?
- The proposed model
- Calpain summary
- Answers to the questions
- Acknowledgements
Topics Covered
- Inhaled bacteria activate Ca2+ fluxes in airway cells
- Signal proinflammatory chemokine and cytokine production
- Ca2+ acts as a second messenger to stimulate adjacent cells
- Ca2+ activates proteases to facilitate PMN transmigration
Links
Series:
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Talk Citation
Prince, A. (2009, October 29). Bacterial infection of epithelial signaling [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved October 31, 2024, from https://doi.org/10.69645/LEDL3870.Export Citation (RIS)
Publication History
Financial Disclosures
- There are no financial matters to disclose.