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1. Introduction
2. High resolution structure of ribosomal subunits
3. Elongation cycle of protein synthesis
4. Understanding the ribosome structure and function
5. Large ribosomal subunit: secondary structure
6. Small ribosomal subunit: secondary structure
7. Crown view of the large ribosomal subunit (1)
8. Various domains of the 23S rRNA
9. Various types of tertiary RNA interactions
10. RNA tertiary interactions structural motifs
11. Ribose zipper (RNA-RNA)
12. Classification of ribose zippers
13. Ribose zippers of different types in the 30S subunit
14. The A-minor motif (RNA-RNA interactions)
15. The A-minor motif (1)
16. Types of A-minor motif mediated interactions
17. Loop-helix contacts
18. RNA crosslinking
19. A-minor motifs cluster in patches
20. RNA-metal ion interactions
21. Mg2+ ions and rRNA interaction types
22. Example of G-phosphate Mg2+ interactions
23. Monovalent ions and rRNA interaction types
24. The K-turn motif (RNA-protein)
25. Kink turns in the large ribosomal subunit
26. Archetypal K-turn example
27. Superposition of the eight ribosomal kink turns
28. Ribosomal proteins interact with K-turns
29. Secondary structures of various K-turns
30. Ribosomal proteins
31. Crown view of the large ribosomal subunit (2)
32. Back view of the large ribosomal subunit
33. Proteins belonging to the large ribosomal subunit
34. Ribosomal proteins evolved to exist within rRNA
35. Ribosomal protein L22 contacts all rRNA domains
36. Amino acid composition of ribosomal proteins
37. Charge distribution in ribosomal proteins
38. Surface area buried at the interface
39. Different modes of RNA:protein interactions
40. L18 and S11 have the same RNA binding strategy
41. Alpha/beta fold interacts with RNA
42. Archeal and bacterial ribosomal proteins
43. rRNA expansion segments and ribosomal proteins
44. Implications for ribosmal active sites architecture
45. tRNA binding in the A, P and E sites
46. mRNA-tRNA codon-anticodon interaction
47. Position "1" codon - anticodon interaction
48. The mRNA is stabilized at the kink by a Mg2+ ion
49. The active site of the large ribosomal subunit
50. The large subunit with tRNAs in the active site
51. The A-minor motif (2)
52. P-site tRNA and ribosomal proteins extensions
53. Peptidyl transferase center and metal ions
54. Peptidyl transferase center and protein extensions
55. Internal loop of ribosomal protein (L4)
56. Intricacy of RNA-protein interactions
57. RNA portions in the vicinity of the exit tunnel
58. RNA-RNA interactions during translation

Topics Covered

• X-ray crystallography
• Protein synthesis
• High resolution structure of ribosomal subunits
• RNA motifs and interactions
• RNA protein interactions
• Metal ions stabilizing RNA structure
• Functional implications of RNA/RNA and RNA/protein interactions during protein synthesis

Links

Series:

• The Mechanisms of Ribosome Function

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Ban, N. (2008, May 15). High resolution structure of ribosomal subunits RNA/RNA and protein/RNA interactions [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 19, 2024, from https://hstalks.com/bs/797/.
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Publication History

• Published on May 15, 2008

Financial Disclosures

• Prof. Nenad Ban has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.