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Talk: Stem cells derived from amniotic fluid and placenta (30 min)

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  1. Introduction
  2. Amniotic fluid and placenta
  3. Possible source for alternate stem cells?
  4. Amniotic fluid and placental derived stem cells
  5. 400 human amniotic fluid and placental samples
  6. Clonality confirmed by Southern blot
  7. Preservation of telomere length
  8. Cells had markers consistent with ES
  9. Cells had markers consistent with adult cells (1)
  10. Cells had markers consistent with adult cells (2)
  11. AFS cells show high similarity to ES and iPS
  12. Isolated cells after 250 population doublings
  13. AFS costimulatory molecule expression
  14. hAFS cells inhibit T-cell activation in vitro
  15. The cells are able to form embryoid bodies (EB)
  16. Embryoid bodies microscopy
  17. EB show the presence of 3 germ layers (1)
  18. EB show the presence of 3 germ layers (2)
  19. Immunomodulation
  20. Regenerative medicine application of AFS
  21. Fat differentiation
  22. Bone differentiation of human AFS cells
  23. Application of bone derived cells - printer
  24. Implantation of bone structure in rodent model
  25. Cartilage differentiation
  26. Cardiac differentiation
  27. How cardiac differentiation actually works
  28. Co-culture of NRCs with hAFS cells
  29. Co-culture of calcein preloaded NRCs with hAFS
  30. Endothelial differentiation
  31. Liver differentiation
  32. Lung differentiation
  33. hAFSC express endogenous PDPN
  34. Clara cells damage through naphthalene injury
  35. hAFSC did not show fusion
  36. Amniotic fluid stem cells in the kidney
  37. AFSC for renal therapy
  38. Kidney injury model
  39. Functionality of kidney injury model
  40. Necrotizing enterocolitis (NEC)
  41. NEC model
  42. 72 hrs post-injection (NEC model)
  43. PCR analysis
  44. EGFP amplification on genomic DNA
  45. Intestinal macroscopic assessment
  46. Intestinal microscopic assessment
  47. AFSC display hematopoietic activity
  48. Amniotic fluid & placental stem cells: clinical utility
  49. Summary
  50. Publication history
  51. Acknowledgements
  52. END
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DETAILED SLIDE INDEX

  1. 1. Introduction
  2. 2. Amniotic fluid and placenta
  3. 3. Possible source for alternate stem cells?
  4. 4. Amniotic fluid and placental derived stem cells
  5. 5. 400 human amniotic fluid and placental samples
  6. 6. Clonality confirmed by Southern blot
  7. 7. Preservation of telomere length
  8. 8. Cells had markers consistent with ES
  9. 9. Cells had markers consistent with adult cells (1)
  10. 10. Cells had markers consistent with adult cells (2)
  11. 11. AFS cells show high similarity to ES and iPS
  12. 12. Isolated cells after 250 population doublings
  13. 13. AFS costimulatory molecule expression
  14. 14. hAFS cells inhibit T-cell activation in vitro
  15. 15. The cells are able to form embryoid bodies (EB)
  16. 16. Embryoid bodies microscopy
  17. 17. EB show the presence of 3 germ layers (1)
  18. 18. EB show the presence of 3 germ layers (2)
  19. 19. Immunomodulation
  20. 20. Regenerative medicine application of AFS
  21. 21. Fat differentiation
  22. 22. Bone differentiation of human AFS cells
  23. 23. Application of bone derived cells - printer
  24. 24. Implantation of bone structure in rodent model
  25. 25. Cartilage differentiation
  26. 26. Cardiac differentiation
  27. 27. How cardiac differentiation actually works
  28. 28. Co-culture of NRCs with hAFS cells
  29. 29. Co-culture of calcein preloaded NRCs with hAFS
  30. 30. Endothelial differentiation
  31. 31. Liver differentiation
  32. 32. Lung differentiation
  33. 33. hAFSC express endogenous PDPN
  34. 34. Clara cells damage through naphthalene injury
  35. 35. hAFSC did not show fusion
  36. 36. Amniotic fluid stem cells in the kidney
  37. 37. AFSC for renal therapy
  38. 38. Kidney injury model
  39. 39. Functionality of kidney injury model
  40. 40. Necrotizing enterocolitis (NEC)
  41. 41. NEC model
  42. 42. 72 hrs post-injection (NEC model)
  43. 43. PCR analysis
  44. 44. EGFP amplification on genomic DNA
  45. 45. Intestinal macroscopic assessment
  46. 46. Intestinal microscopic assessment
  47. 47. AFSC display hematopoietic activity
  48. 48. Amniotic fluid & placental stem cells: clinical utility
  49. 49. Summary
  50. 50. Publication history
  51. 51. Acknowledgements
  52. 52. END

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TALK'S CITATION

Atala, A. (2014), "Stem cells derived from amniotic fluid and placenta", in Atala, A. (ed.), Stem Cells: Recent advances in understanding and utilizing, The Biomedical & Life Sciences Collection, Henry Stewart Talks Ltd, London (online at http://hstalks.com/?t=BL1753697)

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ABOUT THIS TALK

Speaker(s)

Prof. Anthony Atala Show Biography

SPEAKER BIOGRAPHY

Prof. Anthony Atala – Wake Forest Institute for Regenerative Medicine, USA

Anthony Atala, MD, is the Director of the Wake Forest Institute for Regenerative Medicine, and the W.H. Boyce Professor and Chair of the Department of Urology at Wake Forest University. Dr. Atala works with several journals and serves in various roles, including Editor-in-Chief of Stem Cells - Translational Medicine and Current Stem Cell Research and Therapy; as Associate Editor of Tissue Engineering and Regenerative Medicine, The Journal of Rejuvenation Research, and Nanotechnology in Engineering and Medicine; and as Editorial Board member of Expert International Journal of Stem Cells and Stem Cell Review Letters. Dr. Atala is a recipient of many awards, including the Christopher Columbus Foundation Award, the Samuel D. Gross Prize, the Barringer Medal and the Gold Cystoscope award. Dr. Atala was named by Scientific American as a "Medical Treatments Leader of the Year" for his contributions to the fields of cell, tissue and organ regeneration. He is the editor of twelve books, including Principles of Regenerative Medicine, Foundations of Regenerative Medicine, Methods of Tissue Engineering, and Minimally Invasive Urology. He has published more than 300 journal articles and has applied for or received over 200 national and international patents.

Publication Date

March, 2014

Topics Covered

Introduction to the amniotic fluid and placenta... more

TOPICS COVERED IN THIS TALK

  • Introduction to the amniotic fluid and placenta
  • Collecting stem cells from the amniotic fluid or placenta
  • Cell markers in amniotic fluid or placenta derived stem cells
  • Amniotic fluid or placenta derived stem cells are able to form embryoid bodies
  • Uses of amniotic fluid or placenta derived stem cells: Immunomodulation
  • Uses of amniotic fluid or placenta derived stem cells: Regenerative medicine
  • Amniotic fluid or placenta derived stem cells can be differentiated into a variety of cell types
  • Amniotic fluid stems cells in the kidney
  • Necrotizing enterocolitis; a model and treatment with amniotic fluid stems cells
  • Clinical uses of amniotic fluid or placenta derived stem cells

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