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Printable Handouts
Navigable Slide Index
- Introduction
- Biophysics of Ca signaling
- Extracellular and intracellular calcium sources
- Diffusion limits the spread of Ca ions in a cell
- Ca diffusion in the cytosol is slowed by Ca buffers
- Spatial aspects of Ca signaling
- Ca feedback on the IP3 receptor
- Ca induced Ca release propagates Ca waves
- Experimental methods
- Fluorescence microscopy
- Conventional fluorescence image of pollen grain
- Confocal sections through pollen grain
- Caged IP4
- Line-scanning confocal microscope - the system
- Line-scanning confocal microscope - results
- The experimental system: oocyte of X. laevis
- 'Elementary' calcium release events
- 'Local' Ca "puffs" in the Xenopus oocyte
- Linescan imaging of puffs
- Triggering of Ca waves by puffs
- Model of local and global IP3-evoked Ca signals
- The spread of Ca during elementary events
- Ca signals show a continuum of sizes
- Kinetics of puffs
- Puff occurrence is stochastic
- Saltatory propagation of Ca waves between puffs
- Triggering of calcium waves by puffs
- Method for rapidly changing [IP3] and [Ca] (1)
- Both photolysis and UV zap simultaneously
- Ca amount in a puff is too small to trigger a wave
- Role of puffs in setting frequency of Ca waves
- Puffs occur between, and trigger, Ca waves
- The regular periodicity of Ca waves
- Failure of puffs to trigger a wave
- Wave triggering depends on Ca accumulation
- Focal wave initiation sites are more IP3-sensitive
- Ca buffers affect coupling between puff sites
- Ca buffering proteins
- 'Slow' vs. 'fast' Ca buffers
- Conclusions
- Why are Ca channels organized in clusters?
- Acknowledgements
Topics Covered
- Calcium as a life and death cellular messenger
- Sequestration and liberation of calcium from the endoplasmic reticulum
- Calcium release through inositol trisphosphate receptors and modulation by calcium
- Optical techniques to visualize and stimulate intracellular calcium signals
- 'Elementary' calcium signals through single channels and clusters of channels
- Generation of periodic calcium waves by stochastic elementary events
- Modulation of calcium signals by calcium binding proteins
- The hierarchical organization of cellular calcium signals
Talk Citation
Parker, I. (2007, October 1). Spatial and temporal aspects of cellular calcium signals: 'elementary' signals and waves [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved August 31, 2024, from https://doi.org/10.69645/NYDX3531.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Ian Parker has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.
Spatial and temporal aspects of cellular calcium signals: 'elementary' signals and waves
A selection of talks on Cell Biology
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