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1. Introduction
2. Biophysics of Ca signaling
3. Extracellular and intracellular calcium sources
4. Diffusion limits the spread of Ca ions in a cell
5. Ca diffusion in the cytosol is slowed by Ca buffers
6. Spatial aspects of Ca signaling
7. Ca feedback on the IP3 receptor
8. Ca induced Ca release propagates Ca waves
9. Experimental methods
10. Fluorescence microscopy
11. Conventional fluorescence image of pollen grain
12. Confocal sections through pollen grain
13. Caged IP4
14. Line-scanning confocal microscope - the system
15. Line-scanning confocal microscope - results
16. The experimental system: oocyte of X. laevis
17. 'Elementary' calcium release events
18. 'Local' Ca "puffs" in the Xenopus oocyte
19. Linescan imaging of puffs
20. Triggering of Ca waves by puffs
21. Model of local and global IP3-evoked Ca signals
22. The spread of Ca during elementary events
23. Ca signals show a continuum of sizes
24. Kinetics of puffs
25. Puff occurrence is stochastic
26. Saltatory propagation of Ca waves between puffs
27. Triggering of calcium waves by puffs
28. Method for rapidly changing [IP3] and [Ca] (1)
29. Both photolysis and UV zap simultaneously
30. Ca amount in a puff is too small to trigger a wave
31. Role of puffs in setting frequency of Ca waves
32. Puffs occur between, and trigger, Ca waves
33. The regular periodicity of Ca waves
34. Failure of puffs to trigger a wave
35. Wave triggering depends on Ca accumulation
36. Focal wave initiation sites are more IP3-sensitive
37. Ca buffers affect coupling between puff sites
38. Ca buffering proteins
39. 'Slow' vs. 'fast' Ca buffers
40. Conclusions
41. Why are Ca channels organized in clusters?
42. Acknowledgements

Topics Covered

• Calcium as a life and death cellular messenger
• Sequestration and liberation of calcium from the endoplasmic reticulum
• Calcium release through inositol trisphosphate receptors and modulation by calcium
• Optical techniques to visualize and stimulate intracellular calcium signals
• 'Elementary' calcium signals through single channels and clusters of channels
• Generation of periodic calcium waves by stochastic elementary events
• Modulation of calcium signals by calcium binding proteins
• The hierarchical organization of cellular calcium signals

Links

Series:

• Calcium Signaling I

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Parker, I. (2007, October 1). Spatial and temporal aspects of cellular calcium signals: 'elementary' signals and waves [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 18, 2025, from https://doi.org/10.69645/NYDX3531.
Export Citation (RIS)

Publication History

• Published on October 1, 2007
• Archived on February 25, 2021

Financial Disclosures

• Prof. Ian Parker has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.