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microRNA function in stem cells - play sample talk extract

Prof. Hannele Ruohola-Baker - University of Washington, USA

Hannele Ruohola-Baker is a Professor of Biochemistry and Genome Sciences at the University of Washington. She received her BA from the University of Helsinki, Finland, her PhD from Yale University and the University of Helsinki and she completed her postdoc work at the University of California, San Francisco. She has won many awards including the Tietze Award in 2007. The research in her lab focuses on two issues: miRNA function in stem cells and Drosophila as a model for human diseases.

Talk Online Publication: Sep 2008

How to cite this talk:
Ruohola-Baker, H. (2008), "microRNA function in stem cells", in Bier, E. (ed.), The Legacy of Drosophila Genetics: From 'defining the gene' to 'analyzing genome function', The Biomedical & Life Sciences Collection, Henry Stewart Talks Ltd, London (online at http://hstalks.com/bio)

Direct talk access link:
http://hstalks.com/lib.php?t=HST34.1753_1_2&c=252

TOPICS COVERED IN MICRORNA FUNCTION IN STEM CELLS

microRNAs | Stem cells | Stem cell cell cycle | Stem cell checkpoints | p21 | hESCs | Stem cell self-renewing division | Notch | Niche

    DETAILED SLIDE INDEX

1. Introduction
2. Stem cells
3. Bristlecone pine
4. Control of stem cell division
5. microRNA and stem cells in model organisms
6. microRNA function in stem cells
7. Characteristics of microRNAs
8. Mutating Dicer and Drosha in Drosophila GSCs
9. Dcr-1 mutants
10. Germ-line stem cells location
11. GSC maintenance and self renewal
12. Notch pathway activation induces extra niche cells
13. GSC maintenance: niche to GSC signaling
14. Drosophila Dicer is required for GSC maintenance
15. Dicer and Mad are required for GSC maintenance
16. Analysis of microRNAs expression in GSC
17. miR-7, miR-8 and bantam are expressed in GSCs
18. Which microRNA is required in GSC?
19. Bantam is required for GSC self-renewal
20. Signaling pathways in GSC self-renewal: summary
21. microRNA control of stem cell division
22. Dicer and Mad in Drosophila larval/pupal clones
23. Drosophila oogenesis
24. Drosophila oogenesis in Dcr-1 mutants
25. Dcr1-mutant stem cells stop dividing!
26. Dcr1-mutant stem cells halt in G1/S transition
27. Is Dacapo a direct target of microRNAs?
28. Analysis of Dacapo regulation
29. Dacapo promoter is not responsive to Dicer
30. Do microRNAs directly regulate Dap 3'UTR?
31. Dap 3'UTR is responsive to miRNAs in GSCs
32. Dap 3'UTR is responsive to miR-7 and -309
33. microRNA in Drosophila GSC: summary (1)
34. microRNA in Drosophila GSC: summary (2)
35. microRNA function in hESCs
36. Dicer and Drosha knock-down
37. Lentiviral construct
38. Confirm knockdown by QPCR
39. mRNA profiling in KD cells
40. Drosha and Dicer KD hESC(H1)- super stem cells
41. Dicer KD cells have higher level of Oct4 protein
42. Division rate of Dicer and Drosha KD hESCs(H1)
43. Cell cycle status of the mutant hESCs(H1)
44. p21 protein is elevated in Dicer KD cells
45. microRNA profiling in H7, H1 and HSF6 cells
46. hESCs express a unique set of microRNAs
47. Downregulation of microRNAs upon differentiation
48. Differentiating vs. undifferentiated ES cells
49. Rescue of Dicer KD phenotypes
50. Summary
51. Acknowledgements
52. END