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mRNA Capping
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    SPEAKER(S)

Prof. Aaron Shatkin - University of Medicine and Dentistry of New Jersey and the Center for Advanced Biotechnology and Medicine, USA

Aaron Shatkin majored in chemistry at Bowdoin College and trained at Rockefeller University in microbiology with E.L. Tatum. In 1986, he was appointed the director of the Center for Advanced Biotechnology and Medicine, jointly administered by Rutgers University, where he is Professor of Molecular Biology and by UMDNJ-Robert Wood Johnson Medical School where he is Professor of Molecular Genetics, Microbiology and Immunology. His lab's findings have provided many important insights into how gene expression is regulated in eukaryotic cells and viruses. He was the founding editor of Molecular and Cellular Biology and is the author of more than 200 scientific publications. He has received several honors including a DSc (Hon) from Bowdoin College and the 2003 Award for Distinguished Research in the Biomedical Sciences.

Talk Online Publication: Oct 2007

TOPICS COVERED IN MRNA CAPPING

Modification of mRNA 5' ends by addition of a m7G cap structure - Capping for viability - Conservation in yeast to humans - Structure of caps and capping enzymes - Mechanism of cap synthesis - Protein-protein interactions that link capping to events in transcription, transport and translation - siRNA knockdown experiments

How to cite this talk:
Shatkin, A. (2007), "mRNA Capping", in Tsonis, P. (ed.), From DNA to Proteins: The Multiple Levels of Regulation , The Biomedical & Life Sciences Collection, Henry Stewart Talks Ltd, London (online at http://hstalks.com/bio)

Direct talk access link:
http://hstalks.com/lib.php?t=HST6.1116_1_2&c=252

    DETAILED SLIDE INDEX

1. Introduction
2. mRNA 5' cap structure
3. mRNA cap functions
4. Cap binding and translation initiation
5. Cap synthesis
6. Capping enzyme structure
7. Complementation of S.cerevisiae lacking GT
8. Recruitment of mRNA cap enzymes by P-CTD
9. P-CTD enhances guanylylation and cap formation
10. hSPT5 is a transcription elongation factor
11. SPT5 enhances guanylylation and capping
12. DSIF overcome FCP1-mediated capping decrease
13. Capping enzyme reverses repression by NELF
14. Importin-alfa
15. Imp-alfa increases MT activity
16. MT selectively binds GpppG-RNA
17. Imp-alfa enhances MT targeting to GpppG-RNA
18. Importation of nuclear proteins
19. Imp-beta blocks Imp/MT/RNA formation
20. RanGTP counteracts Imp-beta effects
21. Capping modulation
22. MT regions required for Imp-alfa binding
23. MT sequence localizes proteins to nucleus
24. MT NLS narrowed
25. Alternative NLSs in MT
26. Nuclear localized MT binds pol II
27. Capping enzyme RNAi is lethal in C.elegans
28. SiRNA inhibits expression of capping enzymes
29. Apoptosis - cleavage of caspase-3
30. RNA interference induces apoptosis
31. Inducible CE RNAi causes apoptosis
32. Summary
33. Thanks
34. END