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Mechanisms of Transcription: The Eukaryotic Pre-Initiation Complex
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    SPEAKER(S)

Prof. Michael Hampsey - University of Medicine and Dentistry of New Jersey, USA

Michael Hampsey is a native of the Finger Lakes region of New York State. He earned his BS degree in chemistry at the State University of New York at Geneseo and PhD in biochemistry at Purdue University. Following postdoctoral training in yeast genetics at the University of Rochester he was on the faculty at Louisiana State University Medical School in Shreveport. He is currently Professor of Biochemistry at the University of Medicine and Dentistry of New Jersey and member of the UMDNJ - Master Educators' Guild. He lives with his wife Eileen and their three children in Metuchen, New Jersey.

Talk Online Publication: Oct 2007

TOPICS COVERED IN MECHANISMS OF TRANSCRIPTION: THE EUKARYOTIC PRE-INITIATION COMPLEX

Overview of RNA polymerase II transcription - Core promoter elements - Proximal promoters - Enhancers and silencers - General transcription factors - Activators and repressors - Coactivator complexes - RNA polymerase II structure and function - Mechanisms of transcription initiation - Steroid hormone receptors - Coactivator complexes, including mediator and TFIID - Chromatin remodellers including SWI/SNF - Covalent histone modifiers, including histone acetyltransferases, methyltransferases and deacetylases

How to cite this talk:
Hampsey, M. (2007), "Mechanisms of Transcription: The Eukaryotic Pre-Initiation Complex", in Tsonis, P. (ed.), From DNA to Proteins: The Multiple Levels of Regulation , The Biomedical & Life Sciences Collection, Henry Stewart Talks Ltd, London (online at http://hstalks.com/bio)

Direct talk access link:
http://hstalks.com/lib.php?t=HST6.1122_1_2&c=252

    DETAILED SLIDE INDEX

1. Introduction
2. Eukaryotic RNA polymerases I, II and III
3. Introduction to transcription
4. Transcriptional complexity
5. Fundamental questions
6. Pol II machinery: overview of topics
7. DNA sequence elements
8. The RNA polymerase "promoter"
9. Pol II machinery: the core promoter
10. Pol ll core promoter elements
11. Pol ll machinery: the proximal promoter
12. Proximal promoter element: CpG islands
13. Summary: core promoter elements
14. Pol ll machinery: enhancer and silencers
15. Enhancer elements
16. The enhanceosome
17. UAS elements (yeast)
18. Silencer elements
19. URS elements (yeast)
20. Pol ll machinery: the GTFs
21. Discovery of the GTFs
22. The Pol ll GTFs
23. GTF functions
24. Assembly of the Pol ll PIC
25. The GTFs: analogous to sigma factor
26. TFIID
27. TBP
28. TAFs
29. TAF functions
30. TFIID 3D structure
31. Class ll promoters
32. TBP-less TAF complexes
33. TBP-like factors (TLFs)
34. TFIIA
35. TFIIB
36. The "finger" domain
37. TFIIF
38. TFIIE
39. TFIIH
40. TFIIH: subunit structure
41. Key questions: how does Pol ll identify a gene?
42. Pol II machinery: Pol ll itself
43. Pol II
44. Yeast Pol II 3D structure
45. Yeast RNA Pol II - 12 subunit structure
46. A marvellous machine for making messages
47. The Pol II CTD
48. Regulation of the Pol II CTD
49. Pol II machinery: activators and repressors
50. Transcription activators: bipartite structure
51. Nuclear receptor family
52. Activator specificity
53. Pol II machinery: coactivators
54. Mechanisms of activation
55. Coactivators of transcription
56. Transcriptional repression
57. Another repression mechanism
58. Poll ll machinery: mechanism of initiation
59. Pol ll transcription cycle
60. The Pol ll scaffold
61. How does Pol ll initiate transcription?
62. How is transcription activated or repressed?
63. Unanswered questions
64. Scheme of key elements and factors
65. Appendix
66. END