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1. Introduction
2. Schematic structure of the ribosome (1964)
3. Text book ribosome structure
4. Structure of the ribosome with tRNA and mRNA
5. Venki Ramakrishnan
6. Model of the large ribosomal subunit
7. Nenad Ban
8. Poul Nissen
9. Structure of the ribosomal large subunit
10. How porous is the interior of the the ribosome?
11. The interior of the large subunit
12. Slices through the ribosome
13. Escape radius of the tunnel
14. Branches in the exit tunnel
15. A view of the tunnel at different radii
16. Map of the exit tunnel
17. Inside the tunnel
18. Tunnel visions
19. Source of ribosome's catalytic power
20. Sources of enzyme catalytic power
21. Structures of states in a process are important
22. Many snapshots are required for understanding
23. Peptydil transferase reaction (1)
24. Jeff Hansen
25. Analogue of the transition state
26. Binding of Yarus transition state analogue
27. Active site is composed of tightly packed RNA
28. The substrate binding region is highly conserved
29. Proximity of proteins to the active site
30. No proteins found within 18A from active site
31. The ribosome is a ribozyme
32. Martin Schmeing
33. Activity of large subunit crystals
34. Peptydil transferase reaction (2)
35. Results of peptydil transferase assay
36. Peptid-bond formation site
37. Peptid-bond formation site with substrate analogue
38. Prevention of peptydil tRNA hydrolysis
39. Induced fit
40. Stimulation of P-site fMet-tRNA deacylation
41. Occluding water molecules from attack positions
42. ChPmn-CCApcb-peptidyl transferase structure
43. CChPmn-CCApcb-peptidyl transferase structure
44. DCA-peptidyl transferase structure
45. Conformational changes induced by substrate (1)
46. Conformational changes induced by substrate (2)
47. The pre-reaction ground state
48. Effect of mutations in A2486
49. Effect of 2-OH removal from A76 of the P-site
50. Possible role for 2-OH on A76 of the P-site
51. Possible activation of 2-OH of A76 by a metal ion
52. Interaction of 2-OH of A76 with solvent molecule
53. No magnesium ions are coordinated to A76 2-OH
54. Metal ion not bound to the 2'OH of the P-site A76
55. Stabilization of tetrahedral carbon transition state
56. Transition state oxyanion points away from A2486
57. The oxyanion hole is a water molecule
58. Contributors to the ribosome's catalytic power
59. E-site proteins of eubacteria and archaea
60. Location of E-site relative to A and P-sites
61. RNA in the E-site
62. Conformation of CCA in the E-site
63. Protein component of the E-site is not conserved
64. CCA binding to T.th. 70S versus H.ma. 50S
65. CCA and 13-deoxytedanolide binding to H.ma. 50S
66. Summary of CCA/13-deoxytedanolide binding
67. The peptidyl transferase reaction (3)
68. Acknowledgements

Topics Covered

• Overview of protein synthesis by the ribosome
• Nature of the polypeptide exit tunnel in the large subunit
• The ribosome is a ribozyme
• Substrate induced fit at the peptidyl transferase center
• Proton shuttle mechanism and transition state stabilization
• Substrate interactions at the large subunit E-site
• Movie of the peptidyl transferase reaction

Links

Series:

• The Mechanisms of Ribosome Function

Categories:

• Biochemistry
• Cell Biology

Talk Citation

Steitz, T. (2008, May 15). The structural basis for how the large ribosomal subunit catalyses peptide bond formation [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved August 31, 2024, from https://doi.org/10.69645/XPYR2559.
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Publication History

• Published on May 15, 2008

Financial Disclosures

• Prof. Thomas Steitz has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.