We noted you are experiencing viewing problems
-
Check with your IT department that JWPlatform, JWPlayer and Amazon AWS & CloudFront are not being blocked by your network. The relevant domains are *.jwplatform.com, *.jwpsrv.com, *.jwpcdn.com, jwpltx.com, jwpsrv.a.ssl.fastly.net, *.amazonaws.com and *.cloudfront.net. The relevant ports are 80 and 443.
-
Check the following talk links to see which ones work correctly:
Auto Mode
HTTP Progressive Download Send us your results from the above test links at access@hstalks.com and we will contact you with further advice on troubleshooting your viewing problems. -
No luck yet? More tips for troubleshooting viewing issues
-
Contact HST Support access@hstalks.com
-
Please review our troubleshooting guide for tips and advice on resolving your viewing problems.
-
For additional help, please don't hesitate to contact HST support access@hstalks.com
We hope you have enjoyed this limited-length demo
This is a limited length demo talk; you may
login or
review methods of
obtaining more access.
Printable Handouts
Navigable Slide Index
- Introduction
- The central dogma
- tRNA is the central player in translation
- The probability of errors in protein synthesis
- Selectivity and transition state
- The genetic code
- Decoding - selection of the correct tRNA
- The bacterial ribosome
- Antibiotics change the fidelity of the ribosome
- Fidelity mutations far from the anticodon
- Selection of aa-tRNA by the ribosome (1)
- Problems with the tRNA selection scheme
- Selection of aa-tRNA by the ribosome (2)
- The atomic structure of the 30S subunit
- First clue of how decoding might work
- Testing the decoding model
- Induced fit on tRNA binding
- Minor groove recognition
- Near-cognate Leu(II) ASL in the 30S
- Structures with cognate and near-cognate ASL
- Binding of Leu(II) tRNA
- Tautomers and base pairs
- An unusual tautomer at 2nd position?
- 30S closing movement
- Ribosome mutations that affect accuracy
- Energetics of transition to closed form
- Domain closure leads to tRNA selection
- tRNA orientations
- TC loop / tRNA summary
- An integrated view of decoding
- Base pairing and fidelity
- Synchrotrons
- Acknowledgements
Topics Covered
- tRNA: the central player in translation
- Selectivity and transition state
- Decoding: selection of the correct tRNA
- The bacterial ribosome
- Antibiotics change the fidelity of the ribosome
- Selection of aa-tRNA by the ribosome
- The atomic structure of the 30S subunit
- Testing the decoding model
- Minor groove recognition of base pairing geometry
- Tautomers and base pairs
- Streptomycin
- Base pairing and fidelity
Talk Citation
Ramakrishnan, V. (2008, May 15). Structural insights into decoding of mRNA by the ribosome [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved August 31, 2024, from https://doi.org/10.69645/NWAT9316.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Venki Ramakrishnan has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.