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1. Introduction
2. Hp associated diseases
3. Size variation of CagA
4. The structure of the cagA gene
5. VacA and CagA association
6. CagA is present in a subset of strains
7. cag is a pathogenicity island of Hp
8. An hotspot for ISs within cag
9. Type I and Type II strains of Hp
10. Transition from commensal to pathogen
11. cag genes are encoding a Type IV system
12. Anatomy of the cag secretion system
13. The structure of the Type IV core
14. Type III and Type IV secretion systems
15. CagA is translocated into the host cells
16. CagA is Tyr-phosphorylated by the c-Src kinase
17. Hp on the top of red blood cell
18. In vitro delivery of CagA using red blood cells
19. Ghosts formation after contacts with Hp
20. The contacts with Hp cause polymerization
21. Appendages induced upon contacts
22. Hp in a biopsy of gastric mucosa
23. Hp distributed on intercellular junctions
24. ZO1 ectopic expression
25. CagA targets the apical junctional complex
26. The apical junctional complex structure
27. CagA activity in polarized epithelial cells
28. Cells expressing CT-CagA elongate
29. Cells elongation
30. Neuron-like movements
31. Intercellular junction
32. C-terminus: elongation properties
33. CT-CagA and NT-CagA staining
34. NT-CagA localizes to the AJC
35. MDCK expressing NT-CagA
36. Elongation and localization and something else?
37. Shrinking process due to CagA action
38. MDCK apical surface
39. E-cadherin re-distribution
40. Loss of cell polarity
41. N and C-terminal portions do not alter polarity
42. CagA induces loss of cell polarity
43. MDCK expressing full-length CagA
44. Only full-length CagA causes migration
45. Anti-apoptotic effect after CagA expression
46. CagA-dependent invadopodia formation
47. Actin signal across BM
48. Invasion process is protease-dependent
49. MMP14 is expressed in CagA dependant manner
50. Summary
51. Possible mechanisms for CagA activity
52. PAR1 interacts with CagA
53. A stable MDCK line expressing CagA
54. A new protein associated with CagA
55. Tumor suppressors targeting
56. Ways of bacteria to contaminate cells
57. Collaborators

Topics Covered

• Helicobacter pylori: a chronic pathogen with lifelong association with the host
• The cag pathogenicity island encoding the Type IV secretion system and the CagA effector molecule
• CagA is translocated into the host cells and is tyrosine-phosphorylated by the c-Src kinase
• CagA affects the host cell terminal differentiation program
• CagA is associated with tumorigenic conversion acting simultaneously on two major pathways

Links

Series:

• Molecular Basis of Bacterial Infection

Categories:

• Cancer
• Microbiology

Therapeutic Areas:

• Oncology

Talk Citation

Covacci, A. (2010, July 15). From CagA to CagA: the metamorphoses of the Helicobacter pylori's cytotoxin-associated gene A into the cancer-associated gene A [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 24, 2024, from https://hstalks.com/bs/1715/.
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Publication History

• Published on May 20, 2010
• Updated on July 15, 2010

Financial Disclosures

• Dr. Antonello Covacci has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.