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1. In vitro study of drug metabolism
2. Lecture overview (1)
3. Lecture overview (2)
4. Subcellular fractionation
5. Hepatocyte isolation
6. Classes of drug metab. enzymes
7. Location of metabolic enzymes
8. Enzyme cofactor requirements
9. Conducting in vitro assays
10. Performing assays - aspects (1)
11. Performing assays - aspects (2)
12. Performing assays - aspects (3)
13. Common applications
14. Metabolite formation kinetics
15. BFC, BQ, CYP1A2 and CYP3A4
16. Metabolic stability
17. Metabolic stability in humans
18. Reaction phenotyping
19. Indep. experimental approaches
20. cDNA-expressed enzymes
21. Correlation approach
22. Enzyme selective inhibitors
23. Inhibitor depletion
24. CYP probe substrates/inhibitors
25. Enzyme inhibition analysis
26. CYP enzymes for drug metabolism
27. Types of enzyme inhibition
28. In vitro competitive inhibition
29. Layout for IC50
30. Example IC50 curves
31. Substrate dependent differences
32. Mechanism-based inhibition
33. Measuring MBI parameters
34. Effect of pre-incubation w/ NADPH
35. Enzyme inhibition results
36. Enzyme inhibition in vivo
37. Conclusions
38. Thank you

Topics Covered

• Major classes of DMEs
• Systems for laboratory study
• Practical aspects
• Examples of application
• Obtaining high quality data
• Minimizing artefacts

Links

Series:

• Drug Metabolizing Enzymes

Categories:

• Biochemistry
• Metabolism & Nutrition
• Pharmaceutical Sciences

Therapeutic Areas:

• Cardiovascular & Metabolic

Talk Citation

Crespi, C. (2007, October 1). Laboratory methods for the in vitro study of drug metabolism [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 20, 2024, from https://hstalks.com/bs/680/.
Export Citation (RIS)

Publication History

• Published on October 1, 2007
• Archived on October 29, 2018

Financial Disclosures

• Dr. Charles Crespi has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.