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Navigable Slide Index

1. Introduction
2. Talk outline
3. Interpretation of gene expression data
4. Microarray data analysis
5. The central dogma of molecular biology
6. The potential transcriptome space is n-dimensional
7. Coordinate gene expression (1)
8. Coordinate gene expression (2)
9. The actual transcriptome space
10. Evolution of current microarray technology
11. Northern blots
12. Dot blots: single gene, many tissues
13. Dot blots: many genes, single tissue
14. Microarrays: radioactivity on nylon
15. What's measured by conventional microarrays?
16. Combined analysis of microarray with NRO array
17. The ratio between total RNA and NRO
18. T cell activation model (1)
19. T cell activation model (2)
20. T cell activation model (3)
21. mRNA stabilization and de-stabilization
22. Gene regulation
23. NRO gene expression is captured quickly
24. Confiriming post transcriptional regulation (1)
25. Confiriming post transcriptional regulation (2)
26. NRO assay using biotin labeling
27. Are we getting full length genes?
28. NRO RNA microarray vs. RT-PCR
29. Optimization by a number of parameters
30. Whole cell mRNA vs. nascent gene transcription
31. Over expressed myc in B cells
32. 48 hours with and without myc
33. Total RNA and NRO of cRNA
34. Comparing affimetrix and illumina
35. Regulation by microRNA
36. Effect of a glucorticosteroid on epithelial cell line
37. NRO RNA full genome analysis
38. Acknowledgements

Topics Covered

• Conceptual frameworks for the interpretation of gene expression data
• Microarray data analysis
• The potential transcriptome space is n-dimensional
• Co-ordinate gene expression
• Evolution of current microarray technology
• Northern blots
• Radioactivity on nylon
• Combined analysis of conventional microarray with NRO array
• Jurkat T cell activation
• Global comparison of whole cell mRNA vs. nascent gene transcription

Links

Series:

• Microarrays

Categories:

• Cell Biology
• Methods

Talk Citation

Cheadle, C. (2009, March 30). Simultaneous microarray measurement of nuclear and whole cell changes in gene expression reveals overlapping but distinct patterns of transcriptional regulation [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 23, 2024, from https://hstalks.com/bs/1199/.
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Publication History

• Published on March 30, 2009
• Archived on February 25, 2021

Financial Disclosures

• Dr. Chris Cheadle has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.