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1. Introduction
2. Oxidant generation during exercise
3. Outline of the talk
4. Methods of assessment of oxidative processes
5. Muscle cell culture
6. Intracellualar probes assess ROS activity
7. Intracellular ROS detection using DCFH
8. DCF fluorescence from myotubes
9. Extracellular ROS generated by myotubes
10. Working scheme for ROS generation (1)
11. Rate of change in DCF fluorescence (1)
12. Rate of change in DCF fluorescence (2)
13. ROS genereration in single fibrers
14. Measurment of intracellular ROS in muscle fibres
15. DCF fluorescence from single muscle fibres
16. Working scheme for ROS generation (2)
17. Potential actions of ROS in skeletal muscle
18. Effect of non-damaging contractile activity
19. HSP production in soleus muscles
20. Muscle to contractile activity mediation
21. Comparing the preconditioning effects
22. Comparison of mRNA expression changes
23. Redox-regulated transcription factors
24. Multiple effects of oxidants on NF-kappa B
25. Should we try and prevent ROS-induced effects?
26. Potential protective effects of vitamin E
27. Different ways we use muscles
28. Maximum muscle force after damaging exercise
29. Histological appearance after damaging exercise
30. Muscle glutathione following lengthening
31. Prevention of contraction-induced damage
32. Effect of vitamin E supplementation on CK activity
33. Lack of effect of vitamin E supplementation
34. Number and percentage of damaged fibers
35. Antioxidant intervention studies in exercise
36. Antioxidants modulate muscle response to ROS
37. Muscle SOD/catalase activities after ergometry
38. Muscle HSP60 content following cycle ergometry
39. Effect of vitamin C on muscle HSP content
40. Effect of treatment with H2O2 on HSP content
41. Effect of vitamin E supplementation on adaption
42. Importance of adaptations in HSP
43. Transgenic approach to overexpress HSP70
44. Force generated by wild type muscle
45. Effect of increased content of HSP71
46. Quantification of undamaged muscle
47. Conclusions
48. Tissue responses to ROS: theory consideration
49. Acknowledgements of contributors
50. Cellular pathophysiology group
51. References

Topics Covered

• Assessment of oxidative processes
• ROS in skeletal muscle
• ROS generation
• Measurement of intracellular ROS
• Potential actions of ROS in skeletal muscle
• Effect of non-damaging contractile activity
• HSP production in soleus muscles
• Are adaptations of muscle to contractile activity mediated by oxidants?
• ROS-induced effects on skeletal muscle
• Antioxidant intervention studies
• Muscle SOD and catalase activities
• Muscle HSP60 content
• Vitamin E supplementation

Links

Series:

• Free Radicals and the Oxygen Paradox

Categories:

• Biochemistry

Talk Citation

Jackson, M. (2007, November 1). Exercise and free radical generation by contracting skeletal muscle [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved April 20, 2024, from https://hstalks.com/bs/379/.
Export Citation (RIS)

Publication History

• Published on November 1, 2007
• Archived on May 31, 2018

Financial Disclosures

• Prof. Malcolm Jackson has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.