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1. Introduction
2. Proteins export from the ER
3. Model for evolutionary origin of nucleus and ER
4. "Hawaiian punch" view of the cell
5. Specific protein modifications occurr in the ER
6. Advantages of eukaryotic secretion system
7. Destination of proteins synthesized in the ER
8. Proteins traffic through the Golgi apparatus
9. Main themes to be further discussed
10. COPII vesicle formation involves a cytosolic coat
11. Role of Sec13/31 complex in COPII cage formation
12. Flexibility of the COPII cage
13. Role of Sec23/24 complex in COPII cage formation
14. Cargo proteins contain export signals
15. Induction of ER membrane curvature by Sar1-GTP
16. Sar1 promotes disassembly of the COPII coat
17. Homotypic fusion of COPII vesicles
18. ER proteins are retrieved from the Golgi
19. Transitional ER (tER) sites
20. Cellular distribution of mammalian tER sites
21. Mammalian tER sites location relative to Golgi
22. The ER-Golgi intermediate compartment (ERGIC)
23. Microtubules elimination results in Golgi stacks
24. A yeast model for the tER-Golgi relationships
25. tER and Golgi are adjacent in Pichia
26. How do tER sites proliferate?
27. Pichia tER sites form de novo and fuse
28. A self-organization model for tER site formation
29. Sec16 is a key organizer of tER sites
30. Mammalian cells contain Sec16 homologs
31. Pychia and tER Golgi dynamics are linked
32. Golgi organization in Saccharomyces and Pichia
33. Two yeasts have different tER organization
34. Golgi structure correlates with tER organization
35. The Golgi can be formed de novo from the ER
36. Model for de novo Golgi biogenesis
37. Stability of Golgi cisternae
38. Cisternal maturation vs. stable compartments
39. S. cerevisiae is ideal for observing Golgi cisternae
40. The Saccharomyces Golgi is very dynamic
41. Cisternae clearly mature in Saccharomyces
42. Conclusions

Topics Covered

• Evolutionary origin of the ER
• Role of the ER in membrane biogenesis
• Export from the ER in COPII transport vesicles
• Clustering of COPII components at transitional ER sites
• Relationship between ER export and the Golgi apparatus
• Cisternal maturation of Golgi compartments

Links

Series:

• The Endoplasmic Reticulum

Categories:

• Cell Biology

Talk Citation

Publication History

• Published on October 1, 2007

Financial Disclosures

• Dr. Benjamin Glick has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.