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Printable Handouts
Navigable Slide Index
- Introduction
- 55 years ago
- Studying glycogen phosphorylase
- Degradation of glycogen - adrenaline
- Fight or flight
- Insulin, glycogen synthase and glucagon
- Louis Leloir
- How this research started
- Regulation of phosphorylase
- Photograph of the Coris
- Phosphorylase showed no requirement for AMP
- Edwin Krebs - hesitation
- Cori's preparation of muscle phosphorylase (1)
- Purification of phosphorylase
- Edwin Krebs - Cori's preparation
- Ca2+ contamination is responsible for activation
- Edwin Krebs - ashes experiment
- What was phosphorylated?
- Activation-inactivation cycle of phosphorylase
- Edwin Krebs - JBC reviewers
- Knowledge of phospho proteins in 1950
- Ca2+ involvement in the crude extracts
- Phosphorylase kinase is also phosphorylated
- Sutherland
- cAMP and hormonal control of glycogenolysis
- Ca2+ and muscle contraction
- Single fiber measurement method
- Tension and phosphorylase kinase activity
- Effect of the sarcoplasmic reticulum
- The complete cycle of Ca2+
- Ubiquitous protein phosphorylation
- Phosphorylase was a good protein to work on
- Reciprocal control
- Control of glycogen synthesis and breakdown
- Mechanism of activation of GSK-3
- Not everybody believed in regulation by phos.
- Looking back at the early days
- The world - before and after
- Thank you
Topics Covered
- A historical account of the discovery of reversible protein phosphorylation
- Video clips of the late Edwin Krebs speaking about our early work
- Reversible protein phosphorylation represents one of the most prevalent mechanism by which cellular events are regulated
- Establishment of the first hormonal cascade of successive enzymatic reactions initiated by cAMP
Talk Citation
Fischer, E. (2010, November 30). Phosphorylase and the origin of reversible protein phosphorylation [Video file]. In The Biomedical & Life Sciences Collection, Henry Stewart Talks. Retrieved August 31, 2024, from https://doi.org/10.69645/ROXK9891.Export Citation (RIS)
Publication History
Financial Disclosures
- Prof. Edmond Fischer has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.