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1. Introduction
2. Gene expression induced by extracellular ligand
3. Nuclear organization
4. Chromosomes arrangement in interphase nucleus
5. Visualizing gene expression in living cells
6. Characterization of the U2OS stable cell line
7. The locus is stably integrated in chr. 1p36
8. 2.5 h post-transfection (-) Dox
9. 2.5 h post-transfection + 2.5 h after Dox addition
10. Evidence that RNA is present at transcription site
11. Changes in chromatin structure after activation (1)
12. Changes in chromatin structure after activation (2)
13. 3-D organization of gene expres. 2 h after initiation
14. Nuclear mRNP dynamics consistent with diffusion
15. mRNP particle tracking
16. Tet-ON-VP16-YFP at the locus after Dox addition
17. Tet-ON-VP16-YFP recruited to the locus over time
18. RNA pol. II large subunit recruited to active locus
19. SF2/ASF recruited to active locus
20. CstF-64 recruited to active locus
21. Tri-methyl. histone H3 as a marker for HP1 binding
22. HP1 alpha/beta/gamma enriched at inactive locus
23. Tri-methyl. histone H3 enriched at inactive locus
24. Suv39h1/G9a-L HMTases localize at inactive locus
25. HP1 alpha clears from locus upon transcription (1)
26. HP1 alpha clears from locus upon transcription (2)
27. HP1 alpha clears from locus upon transcription (3)
28. Histone H3 and the H3.3 variant
29. Histone H3.3 enriched at the active locus (1)
30. Histone H3.3 enriched at the active locus (2)
31. Summary (1)
32. Summary (2)
33. Acknowledgements

Topics Covered

• Current understanding of the organization of the mammalian cell nucleus and how this organization impacts on gene expression
• in-vivo studies of gene expression as a result of the concerted processes of transcription, mRNA processing, messenger ribonucleoprotein particle (mRNP) export and translation
• studies of spatial and temporal aspects of critical cellular processes within the context of the living cell
• development of a cell system that allows the visualization of a transcription site and its mRNA and protein products directly in living cells
• evaluation of spatial and temporal changes in chromatin structure, mRNA synthesis and factor association/disassociation during the transition from a transcriptionally inactive to an active state
• understanding of the in-vivo dynamics of gene expression will provide the basis from which to elucidate spatial and/or temporal alterations that occur in cells associated with various disease states

Links

Series:

• Epigenetics
• From DNA to Proteins

Categories:

• Biochemistry
• Cell Biology
• Genetics & Epigenetics

Talk Citation

Publication History

• Published on October 1, 2007
• Archived on May 31, 2018

Financial Disclosures

• Prof. David Spector has not informed HSTalks of any commercial/financial relationship that it is appropriate to disclose.